The Actinobacteriophage Database

Arthrobacter phage MiniMommy

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Detailed Information for Phage MiniMommy
Discovery Information
Isolation Host	Arthrobacter sulfureus ATCC 19098
Former names	MicroMommy
Found By	Cindy Kim
Year Found	2023
Location Found	Los Angeles, CA US
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	34.06096 N, 118.44316 W Map
Discovery Notes	Phage amplification was performed by making an enriched culture. 25 mL of PYCa and 4 mL of 48 hour mixed culture of Arthrobacter was added to the soil sample, which was subsequently incubated at 30ºC for 48 hours. Following filtration by using a 0.22 µm syringe, a 1-plate plaque assay was performed on Arthrobacter species, A. atrocyaneus, A. sulfureus, and A. globiformis to determine if enriched isolate contained phage. Plaques were found to be most prominent on A. sulfureus, which was used as the host bacteria for all subsequent experiments. Team #1 Name: Daddy Gene Blot Team #1 Members: Mulin Li, Diana Reyimjan, Sophie Barden, Niels Dawson, Cindy Kim Team #2 Name: KERMit the Phage Team #2 Members: Esha Chawla, Madeleine Lim, Ryan Hoang, Kirthana Pisipati AL Quarter and Lab section: W23-1C Instructor: Amanda Freise Teaching Assistant: Juliet Stephenson Undergraduate Assistant(s): Alexis and Kylie
Naming Notes	We are daddy and we need a mommy.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 19, 2023
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	795
Genome length (bp)	43680
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGAACTGGCAT
GC Content	66.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	AZ
Subcluster	AZ4
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Schaffner (AZ1) Simpson (AZ1) Skelbel (AZ1) Subaru (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Beaupre (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Giorgio (AZ5) Gumpizza (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) RockScotty (AZ5) Sabourin (AZ5) Sooty (AZ5) SpecialK (AZ5) Stuu (AZ5) Exile (AZ6) Soondubu (AZ6) AinMach (AZ7)
Annotating Institution	University of Pittsburgh at Greensburg
Annotation Status	QC Complete
Plaque Notes	Halo, 1mm diameter
Number of Genes	68
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View MiniMommy_1 (terminase, small subunit) MiniMommy_2 (terminase, large subunit) MiniMommy_3 (portal protein) MiniMommy_4 MiniMommy_5 (scaffolding protein) MiniMommy_6 (major capsid protein) MiniMommy_7 (head-to-tail adaptor) MiniMommy_8 (head-to-tail stopper) MiniMommy_9 MiniMommy_10 (tail terminator) MiniMommy_11 (major tail protein) MiniMommy_12 (tail assembly chaperone) MiniMommy_13 (tail assembly chaperone) MiniMommy_14 (tape measure protein) MiniMommy_15 (minor tail protein) MiniMommy_16 (minor tail protein) MiniMommy_17 (minor tail protein) MiniMommy_18 (minor tail protein) MiniMommy_19 MiniMommy_20 (endolysin) MiniMommy_21 MiniMommy_22 MiniMommy_23 MiniMommy_24 MiniMommy_25 (deoxynucleoside monophosphate kinase) MiniMommy_26 MiniMommy_27 (Cas4 family exonuclease) MiniMommy_28 MiniMommy_29 (HNH endonuclease) MiniMommy_30 MiniMommy_31 MiniMommy_32 (nucleoside deoxyribosyltransferase) MiniMommy_33 (LAGLIDADG endonuclease) MiniMommy_34 (ThyX-like thymidylate synthase) MiniMommy_35 (recombination directionality factor) MiniMommy_36 MiniMommy_37 MiniMommy_38 (Nrdh-like glutaredoxin) MiniMommy_39 (Holliday junction resolvase) MiniMommy_40 MiniMommy_41 (DNA primase/helicase) MiniMommy_42 MiniMommy_43 MiniMommy_44 (DNA polymerase I) MiniMommy_45 MiniMommy_46 MiniMommy_47 MiniMommy_48 (DNA binding protein) MiniMommy_49 (DNA binding protein) MiniMommy_50 MiniMommy_51 MiniMommy_52 (SprT-like protease) MiniMommy_53 MiniMommy_54 MiniMommy_55 MiniMommy_56 MiniMommy_57 MiniMommy_58 MiniMommy_59 MiniMommy_60 MiniMommy_61 MiniMommy_62 MiniMommy_63 MiniMommy_64 MiniMommy_65 MiniMommy_66 MiniMommy_67 — tRNA (tRNA-Trp(cca)) MiniMommy_68 MiniMommy_69 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	184
Pitt Freezer Box Grid#	E4
Available Files
Plaque Picture	Download
GenBank File for Phamerator	Download

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