| Detailed Information for Phage MiniMommy | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Former names | MicroMommy |
| Found By | Cindy Kim |
| Year Found | 2023 |
| Location Found | Los Angeles, CA US |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.06096 N, 118.44316 W Map |
| Discovery Notes | Phage amplification was performed by making an enriched culture. 25 mL of PYCa and 4 mL of 48 hour mixed culture of Arthrobacter was added to the soil sample, which was subsequently incubated at 30ºC for 48 hours. Following filtration by using a 0.22 µm syringe, a 1-plate plaque assay was performed on Arthrobacter species, A. atrocyaneus, A. sulfureus, and A. globiformis to determine if enriched isolate contained phage. Plaques were found to be most prominent on A. sulfureus, which was used as the host bacteria for all subsequent experiments. Team #1 Name: Daddy Gene Blot Team #1 Members: Mulin Li, Diana Reyimjan, Sophie Barden, Niels Dawson, Cindy Kim Team #2 Name: KERMit the Phage Team #2 Members: Esha Chawla, Madeleine Lim, Ryan Hoang, Kirthana Pisipati AL Quarter and Lab section: W23-1C Instructor: Amanda Freise Teaching Assistant: Juliet Stephenson Undergraduate Assistant(s): Alexis and Kylie |
| Naming Notes | We are daddy and we need a mommy. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 19, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 795 |
| Genome length (bp) | 43680 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAACTGGCAT |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAinMach (None) Exile (None) Soondubu (None) Adolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Simpson (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) JasmineDragon (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) Sooty (AZ5) SpecialK (AZ5) |
| Annotating Institution | University of Pittsburgh at Greensburg |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Halo, 1mm diameter |
| Number of Genes | 72 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMiniMommy_1 MiniMommy_2 MiniMommy_3 MiniMommy_4 MiniMommy_5 MiniMommy_6 MiniMommy_7 MiniMommy_8 MiniMommy_9 MiniMommy_10 MiniMommy_11 MiniMommy_12 MiniMommy_13 MiniMommy_14 MiniMommy_15 MiniMommy_16 MiniMommy_17 MiniMommy_18 MiniMommy_19 MiniMommy_20 MiniMommy_21 MiniMommy_22 MiniMommy_23 MiniMommy_24 MiniMommy_25 MiniMommy_26 MiniMommy_27 MiniMommy_28 MiniMommy_29 MiniMommy_30 MiniMommy_31 MiniMommy_32 MiniMommy_33 MiniMommy_34 MiniMommy_35 MiniMommy_36 MiniMommy_37 MiniMommy_38 MiniMommy_39 MiniMommy_40 MiniMommy_41 MiniMommy_42 MiniMommy_43 MiniMommy_44 MiniMommy_45 MiniMommy_46 MiniMommy_47 MiniMommy_48 MiniMommy_49 MiniMommy_50 MiniMommy_51 MiniMommy_52 MiniMommy_53 MiniMommy_54 MiniMommy_55 MiniMommy_56 MiniMommy_57 MiniMommy_58 MiniMommy_59 MiniMommy_60 MiniMommy_61 MiniMommy_62 MiniMommy_63 MiniMommy_64 MiniMommy_65 MiniMommy_66 MiniMommy_67 MiniMommy_68 MiniMommy_69 MiniMommy_70 MiniMommy_71 — tRNA MiniMommy_72 MiniMommy_73 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | E4 |
| Available Files | |
| Plaque Picture | Download |