| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Terry Lee, Chere Petty, Steven Caruso |
| Year Found | 2015 |
| Location Found | Kilauea, HI USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 22.174618 N, 159.435647 W Map |
| Discovery Notes | Sample was collected by Dr. Terry Lee in Kilauea from volcanic soil and provided to Chere Petty, then enriched on Streptomyces griseus griseus ATCC 10137 by SMC. |
| Naming Notes | Nano- for tiny, -odon for tooth = tiny tooth. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1201 |
| Genome length (bp) | 50082 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques 0.5-2.5 mm in d. with rough edges after 48 hrs at 30 C. Phage dim are: capsid l = 48.2 nm, w = 45.6 nm, tail l = 151.3 nm, n = 2. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX344445 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015UMBCNanodonSMC |
| SEA Lysate Titer |
1.3 x 10^9 pfu/ml |
| Date of SEA Lysate Titering |
Nov 18, 2015 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
