Gordonia phage PokyPuppy
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Detailed Information for Phage PokyPuppy
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByElla Eleven
Year Found2021
Location FoundSocorro, NM America
Finding InstitutionNew Mexico Institute of Mining and Technology
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature19°C
GPS Coordinates34.06275 N, 106.903575 W Map
Discovery NotesSoil sample acquired from the side of a pond on the southeast end of NMTech campus. Ground was very wet and covered in pine needles+grass. Collected after scraping away top layer (~1/2 inch below surface.)
Naming NotesI named it Pokey because a bunch of plants poked my socks during collection, but just ‘Pokey’ was taken, so now it’s also my favorite children’s book.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage836
Genome length (bp)77065
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length192 bp
GC Content59.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNew Mexico Institute of Mining and Technology
Annotation StatusIn GenBank
Plaque NotesVery small plaques.
MorphotypeSiphoviridae
Number of Genes104
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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PokyPuppy_1

PokyPuppy_2

PokyPuppy_3

PokyPuppy_4
(DNA binding protein)

PokyPuppy_5

PokyPuppy_6

PokyPuppy_7
(DNA primase)

PokyPuppy_8

PokyPuppy_9
(terminase)

PokyPuppy_10
(portal protein)

PokyPuppy_11
(hydrolase)

PokyPuppy_12

PokyPuppy_13
(capsid maturation protease)

PokyPuppy_14
(capsid decoration protein)

PokyPuppy_15
(major capsid protein)

PokyPuppy_16

PokyPuppy_17

PokyPuppy_18
(head-to-tail adaptor)

PokyPuppy_19

PokyPuppy_20

PokyPuppy_21

PokyPuppy_22
(major tail protein)

PokyPuppy_23 — tRNA
(tRNA-Asn(gtt))

PokyPuppy_24
(tail assembly chaperone)

PokyPuppy_25
(tail assembly chaperone)

PokyPuppy_26
(tape measure protein)

PokyPuppy_27
(minor tail protein)

PokyPuppy_28
(minor tail protein)

PokyPuppy_29
(minor tail protein)

PokyPuppy_30

PokyPuppy_31

PokyPuppy_32

PokyPuppy_33
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

PokyPuppy_34
(lysin A, protease M23 domain)

PokyPuppy_35

PokyPuppy_36

PokyPuppy_37

PokyPuppy_38

PokyPuppy_39
(Cas4 family exonuclease)

PokyPuppy_40

PokyPuppy_41

PokyPuppy_42

PokyPuppy_43

PokyPuppy_44

PokyPuppy_45
(DNA methyltransferase)

PokyPuppy_46

PokyPuppy_47

PokyPuppy_48

PokyPuppy_49
(lysin B)

PokyPuppy_50
(MazG-like nucleotide pyrophosphohydrolase)

PokyPuppy_51
(RusA-like resolvase)

PokyPuppy_52

PokyPuppy_53
(nucleoside deoxyribosyltransferase)

PokyPuppy_54
(DNA polymerase III sliding clamp)

PokyPuppy_55
(DnaQ-like DNA polymerase III subunit)

PokyPuppy_56
(MerR-like helix-turn-helix DNA binding domain protein)

PokyPuppy_57

PokyPuppy_58
(DNA helicase)

PokyPuppy_59

PokyPuppy_60

PokyPuppy_61

PokyPuppy_62
(helix-turn-helix DNA binding domain protein)

PokyPuppy_63

PokyPuppy_64

PokyPuppy_65

PokyPuppy_66

PokyPuppy_67

PokyPuppy_68

PokyPuppy_69

PokyPuppy_70

PokyPuppy_71

PokyPuppy_72

PokyPuppy_73

PokyPuppy_74

PokyPuppy_75

PokyPuppy_76

PokyPuppy_77

PokyPuppy_78

PokyPuppy_79
(HNH endonuclease)

PokyPuppy_80

PokyPuppy_81

PokyPuppy_82

PokyPuppy_83

PokyPuppy_84

PokyPuppy_85

PokyPuppy_86

PokyPuppy_87
(CobT-like cobalamin biosynthesis protein)

PokyPuppy_88
(AAA-ATPase)

PokyPuppy_89

PokyPuppy_90

PokyPuppy_91

PokyPuppy_92

PokyPuppy_93

PokyPuppy_94

PokyPuppy_95

PokyPuppy_96

PokyPuppy_97

PokyPuppy_98

PokyPuppy_99

PokyPuppy_100

PokyPuppy_101

PokyPuppy_102

PokyPuppy_103

PokyPuppy_104

PokyPuppy_105

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456331
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 136
Pitt Freezer Box Grid# F6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload