| Detailed Information for Phage PokyPuppy |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Ella Eleven |
| Year Found | 2021 |
| Location Found | Socorro, NM America |
| Finding Institution | New Mexico Institute of Mining and Technology |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 19°C |
| GPS Coordinates | 34.06275 N, 106.903575 W Map |
| Discovery Notes | Soil sample acquired from the side of a pond on the southeast end of NMTech campus. Ground was very wet and covered in pine needles+grass. Collected after scraping away top layer (~1/2 inch below surface.) |
| Naming Notes | I named it Pokey because a bunch of plants poked my socks during collection, but just ‘Pokey’ was taken, so now it’s also my favorite children’s book. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 836 |
| Genome length (bp) | 77065 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 192 bp |
| GC Content | 59.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | New Mexico Institute of Mining and Technology |
| Annotation Status | In GenBank |
| Plaque Notes | Very small plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON456331 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
136 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
