| Detailed Information for Phage Pumpkins |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Allison Hughes |
| Year Found | 2023 |
| Location Found | Bismarck, ND United States |
| Finding Institution | University of Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 46.72378 N, 100.75427 W Map |
| Discovery Notes | This was found under some dead flower vegetation, and the soil was moist and dark brown with dead vegetation in it. |
| Naming Notes | I chose the name Pumpkin because of my grandma’s pumpkin farm and all the time I have spent there helping out and digging in the fields. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 27, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2667 |
| Genome length (bp) | 43976 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAACCGGCAT |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Mary |
| Annotation Status | In GenBank |
| Plaque Notes | It was a large clear plaque, with a halo of 2 mm, with an average size 13mm for the phages. |
| Morphotype | Siphoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978775 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
169 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
