| Detailed Information for Phage Sam12 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | SH2022 |
| Found By | Samantha Haubner, Devesh Malik |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.436389 N, 79.952778 W Map |
| Discovery Notes | The soil sample was collected on the side of Dawson Street in Pittsburgh PA around 8:30pm on 09/02. The soil was dark in color and damp. While it was cool weather conditions when the soil was collected, it had rained for a majority of the night before. There was a moderate amount of vegetation surrounding the soil. Soil was transported to a refrigerator within 5 minutes of collection and was stored there for 12 hours. |
| Naming Notes | name and favorite number. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1644 |
| Genome length (bp) | 75280 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage titer determined to be 1.0x10^11 pfu/mL. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small (diameter less than 2mm), circular, and clear in appearance. |
| Number of Genes | 104 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889368 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
115 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
