Mycobacterium phage Sonah
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Detailed Information for Phage Sonah
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySavannah Orton and Jonah McGinty
Year Found2020
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.4656 N, 94.7266 W Map
Discovery NotesWe collected a soil sample by digging a hole near flower beds by the Mechanical Engineering Labs at LeTourneau University using a plastic spoon and plastic test tube around 2:45 pm on 08/25/2020. The sample was collected at Latitude: 32.4656 and Longitude: -94.7266 at 93 degrees Fahrenheit and 45% humidity. The soil was moist, dark brown, compact, surrounded by leaves, microorganisms, and other vegetation.
Naming NotesA combination of our first names, Savannah and Jonah.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 17, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage713
Genome length (bp)46414
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes81
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Sonah_1
(terminase small subunit)

Sonah_2
(terminase large subunit)

Sonah_3
(portal protein)

Sonah_4
(capsid maturation protease)

Sonah_5
(scaffolding protein)

Sonah_6
(major capsid protein)

Sonah_7

Sonah_8
(head-to-tail adaptor)

Sonah_9
(head-to-tail stopper)

Sonah_10

Sonah_11

Sonah_12

Sonah_13
(tail terminator)

Sonah_14
(major tail protein)

Sonah_15
(tail assembly chaperone)

Sonah_16
(tail assembly chaperone)

Sonah_17
(tape measure protein)

Sonah_18
(minor tail protein)

Sonah_19
(minor tail protein)

Sonah_20
(minor tail protein)

Sonah_21
(minor tail protein)

Sonah_22
(minor tail protein)

Sonah_23

Sonah_24

Sonah_25

Sonah_26
(lysin A)

Sonah_27
(lysin B)

Sonah_28
(holin)

Sonah_29

Sonah_30
(tyrosine integrase)

Sonah_31
(immunity repressor)

Sonah_32
(excisionase)

Sonah_33

Sonah_34
(antirepressor)

Sonah_35

Sonah_36

Sonah_37

Sonah_38

Sonah_39

Sonah_40

Sonah_41

Sonah_42

Sonah_43

Sonah_44

Sonah_45

Sonah_46
(RecE-like exonuclease)

Sonah_47
(RecT-like DNA pairing protein)

Sonah_48

Sonah_49

Sonah_50
(FtsK-like DNA translocase)

Sonah_51
(RusA-like resolvase)

Sonah_52

Sonah_53

Sonah_54
(DNA methyltransferase)

Sonah_55

Sonah_56
(DNA methyltransferase)

Sonah_57

Sonah_58
(helix-turn-helix DNA binding domain)

Sonah_59

Sonah_60

Sonah_61

Sonah_62

Sonah_63

Sonah_64

Sonah_65

Sonah_66
(helix-turn-helix DNA binding domain)

Sonah_67

Sonah_68

Sonah_69

Sonah_70

Sonah_71

Sonah_72

Sonah_73

Sonah_74

Sonah_75
(ribbon-helix-helix DNA binding domain)

Sonah_76

Sonah_77

Sonah_78

Sonah_79

Sonah_80

Sonah_81
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP358747
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 118
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload