| Detailed Information for Phage Sonah |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Savannah Orton and Jonah McGinty |
| Year Found | 2020 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.4656 N, 94.7266 W Map |
| Discovery Notes | We collected a soil sample by digging a hole near flower beds by the Mechanical Engineering Labs at LeTourneau University using a plastic spoon and plastic test tube around 2:45 pm on 08/25/2020. The sample was collected at Latitude: 32.4656 and Longitude: -94.7266 at 93 degrees Fahrenheit and 45% humidity. The soil was moist, dark brown, compact, surrounded by leaves, microorganisms, and other vegetation. |
| Naming Notes | A combination of our first names, Savannah and Jonah. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 17, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 713 |
| Genome length (bp) | 46414 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP358747 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
D5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
