| Detailed Information for Phage South40 |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Alison Deng and John Georgiades |
| Year Found | 2020 |
| Location Found | St. Louis, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 14°C |
| GPS Coordinates | 38.644689 N, 90.314689 W Map |
| Discovery Notes | Date of collection: 09/29/2020
Time of collection: 10:00 AM CDT
Depth below the surface: 8 cm
Notable nearby objects: picnic table, several trees, a walking path, and a dormitory.
Soil moisture: Dry
Soil quality: Sandy
Humidity: 67%
Atmospheric Pressure: 745.7 mmHg |
| Naming Notes | We decided to name our phage South40 because our soil sample was collected from the forty-acre residential area of our university's campus that we lovingly refer to as the South 40. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 18, 2021 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 187 |
| Genome length (bp) | 51271 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGTCAGTGAT |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are round and medium in size with cloudy edges and a relatively clear center. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ820091 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
