| Detailed Information for Phage Techage |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Isabella Kim |
| Year Found | 2017 |
| Location Found | Blacksburg, VA USA |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.232721 N, 80.409856 W Map |
| Discovery Notes | This phage was found right in front of the Chase Apartments in downtown Blacksburg, VA. During DNA sequencing, it was discovered that Techage was mixed with another phage. Brianna Chop and Cameron Myers separated the two phages in the lab. |
| Naming Notes | Since I found this phage at Virginia Tech, I decided to combine the two names together to create "Techage" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 435 |
| Genome length (bp) | 47094 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | Medium plaques with a clear center and cloudy edge. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919480 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
54 |
| Pitt Freezer Box Grid# |
G4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
