| Detailed Information for Phage Tortellini |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jessica Schipper and Christian Erchinger |
| Year Found | 2013 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.998704 N, 105.252092 W Map |
| Discovery Notes | Under a tree by Stearns West in Williams Village. 9/15/13 9:45pm |
| Naming Notes | Started with the name Apollo and found out the name was already taken. Then we tried to use the name Nagini but, there was copyright issues. So we debated between Tortellini and Flood. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 18, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 154 |
| Genome length (bp) | 49658 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCTGCCGCCCGC |
| GC Content | 65.8% |
| Sequencing Notes | This phage was sequenced as part of a pool of phages. The sequence was matched to the physical sample and name by PCR. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid plaque with light halo, Size(mm): 1.5mm, Incubation time and temperature: Two days in 37°C heat |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX648391 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2013UCBOTortelliniSE |
| SEA Lysate Titer |
7.7x10^10 pfu/mL |
| Date of SEA Lysate Titering |
Nov 8, 2013 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
