| Detailed Information for Phage Zilizebeth |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nzinga Hendricks |
| Year Found | 2017 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.6259 N, 75.3705 W Map |
| Discovery Notes | The soil sample that my phage was extracted from was soil found from behind Drinker House, located on 64 Quad Drive on Lehigh University's campus. The soil was collected January 23rd, 2017, and that particular day it was raining so the soil was damp. |
| Naming Notes | The name for the phage is a mash-up of the second and third letter of my first name (Zi-Nzinga), with lizebeth being a modified spelling of my middle name (which is Elizabeth). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 26, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 672 |
| Genome length (bp) | 48056 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.3% |
| Sequencing Notes | This sample was part of LEHIGH 2017 DOGEMS. Phage was PCR verified.
At position 20346 bp 25% of reads contain an A instead of consensus G.
At position 28321 bp 42% of reads contain a C instead of consensus A.
|
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology of Zilizebeth is the following:
The plaque clearings are very clear (no traces of turbid clearings or rings). This indicates that Zilizbeth follows a lytic cycle. The phage clearings are mixed between small phage clearings (ranging between 0.5-1.0 mm) and large clearings (ranging between 1.5-2.5 mm). The plaques form after 24 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524508 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
51 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
