| Detailed Information for Phage Kari |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deborah Jacobs-Sera |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.443832 N, 79.951982 W Map |
| Discovery Notes | This sample was collected from under a rock in front of the log cabin situated on the lawn of the Cathedral of Learning. |
| Naming Notes | This phage is named after my oldest daughter Karoline, "Kari" for short. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 20260 |
| Genome length (bp) | 47523 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV755393 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
219 |
| Pitt Freezer Box Grid# |
F3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
