| Detailed Information for Phage Neoevie |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Chelsea Smith |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4404 N, 79.959 W Map |
| Discovery Notes | Discovered from direct isolation techniques. Only one enriched isolation test was performed, but no plaques resulted from those methods. |
| Naming Notes | The meaning of "Neoevie" is "new life." Not only is the sequence of each phage finding new understanding of life for researchers, but I see this project as the beginning of a new phase of life for myself. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 266 |
| Genome length (bp) | 73353 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 161 bp |
| GC Content | 59.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The phage appears to be a lytic phage. |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque sizes seems to vary from 1mm-3mm in diameter. |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450436 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.7*10^9pfu/mL |
| Date of SEA Lysate Titering |
Oct 26, 2016 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |