| Detailed Information for Phage Shipwreck |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Audrey Tolbert |
| Year Found | 2012 |
| Location Found | Shippensburg, PA USA |
| Finding Institution | Bucknell University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.01005 N, 77.506444 W Map |
| Discovery Notes | The sample was taken from the surface of rocky, dry soil from a garden. It was most likely topsoil originally but was not recently fertilized. |
| Naming Notes | This name was chosen because the phage was isolated from soil in Shippensburg |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 350 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 48670 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were bullseyes, with a very clear center surrounded by an opaque ring. The size of the opaque ring changed depending on the titer. As the concentration of phage increases, the diameter of the ring decreased. |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU985090 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
59 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
