| Detailed Information for Phage StressBall |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cameron Hartzler and Raul Martinez |
| Year Found | 2018 |
| Location Found | Longview, TX USA |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.464719 N, 94.727251 W Map |
| Discovery Notes | We collected our phage at 9:17 am on 9/27/18. The weather was 62 degrees Fahrenheit and cloudy. The soil was a dark brown color and was originally covered with compositing leaves. We dug in the flower bed behind our science building right next to a patch of monkey grass. We made sure that the hole was about 2 inches deep. |
| Naming Notes | For the first 7 weeks of our adventure, we had no progress whatsoever. Even after we found our phage, one of us (Cameron) still experienced more-than-normal stress levels. He used a stress ball to try and calm his nerves, so we decided to name our phage just that. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 591 |
| Genome length (bp) | 47915 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCTGCCGCCCCGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The average plaque diameter after 5 days of incubation at 37 degrees Celsius is 2.2 mm. The plaques are almost circular, but they have a jagged edge at one end of them. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN908683 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
