| Detailed Information for Phage Teal |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Aliana Rao |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4459 N, 79.9573 W Map |
| Discovery Notes | I discovered my phage outside of the Chevron Science Center at the University of Pittsburgh on August 27, 2018. The soil was collected from a cement planter that was located in semi-shade and it was 87 degrees Fahrenheit at time of collection. |
| Naming Notes | Teal happens to be one of my favorite colors so it only seemed appropriate to name my phage after a color that makes me happy, as my phage does when I see all the work I put in to find it and purify it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1260 |
| Genome length (bp) | 73626 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 149 bp |
| GC Content | 59.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic as proved by lysogen testing which provided clear plaques |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear round and small. They are roughly 1-1.5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878903 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.3 x 10^9 pfu/mL |
| Pitt Freezer Box# |
91 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
