Mycobacterium phage Phlegm
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Detailed Information for Phage Phlegm
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJustin Cicarelli
Year Found2017
Location FoundPhiladelphia, PA United States
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.953976 N, 75.189064 W Map
Discovery NotesDate of sample collection: October 4th, 2017
Time of sample collection: 11:24am
Depth of soil dug: 1.5 inches
Approximate Temperature: 70 degrees Fahrenheit
General Location: Drexel Campus, side entrance of Papadakis Integrated Sciences Building
Sample Site Description: near foliage, dry medium-brown soil
Naming NotesI liked the name phlegm since it has a ph like there is in phage and thought it was funny.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 31, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage537
Genome length (bp)155959
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesTiter: 1.5 x 10^10 pfu/ml
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Phlegm_1

Phlegm_2

Phlegm_3
(nucleotidyl transferase)

Phlegm_4

Phlegm_5

Phlegm_6

Phlegm_7

Phlegm_8

Phlegm_9

Phlegm_10

Phlegm_11

Phlegm_12

Phlegm_13

Phlegm_14

Phlegm_15

Phlegm_16

Phlegm_17

Phlegm_18

Phlegm_19
(histidine triad nucleotide binding protein)

Phlegm_20

Phlegm_21

Phlegm_22

Phlegm_23

Phlegm_24

Phlegm_25

Phlegm_26

Phlegm_27
(HNH endonuclease)

Phlegm_28

Phlegm_29

Phlegm_30

Phlegm_31
(band-7-like membrane protein)

Phlegm_32

Phlegm_33

Phlegm_34

Phlegm_35

Phlegm_36

Phlegm_37

Phlegm_38

Phlegm_39

Phlegm_40

Phlegm_41

Phlegm_42

Phlegm_43

Phlegm_44

Phlegm_45

Phlegm_46

Phlegm_47

Phlegm_48

Phlegm_49

Phlegm_50

Phlegm_51

Phlegm_52

Phlegm_53

Phlegm_54

Phlegm_55

Phlegm_56

Phlegm_57

Phlegm_58

Phlegm_59

Phlegm_60

Phlegm_61

Phlegm_62

Phlegm_63

Phlegm_64
(helix-turn-helix DNA binding domain protein)

Phlegm_65
(helix-turn-helix DNA binding domain protein)

Phlegm_66

Phlegm_67

Phlegm_68

Phlegm_69

Phlegm_70

Phlegm_71

Phlegm_72

Phlegm_73

Phlegm_74

Phlegm_75

Phlegm_76

Phlegm_77

Phlegm_78

Phlegm_79

Phlegm_80

Phlegm_81

Phlegm_82

Phlegm_83

Phlegm_84

Phlegm_85

Phlegm_90

Phlegm_91

Phlegm_92
(portal protein)

Phlegm_93

Phlegm_94

Phlegm_95
(thymidylate synthase)

Phlegm_96

Phlegm_97
(RNA ligase)

Phlegm_98
(HNH endonuclease)

Phlegm_99
(LysM-like endolysin)

Phlegm_100

Phlegm_101

Phlegm_102
(major capsid protein)

Phlegm_103

Phlegm_104

Phlegm_105

Phlegm_106

Phlegm_107

Phlegm_108

Phlegm_109

Phlegm_110

Phlegm_111

Phlegm_112

Phlegm_113

Phlegm_114

Phlegm_115

Phlegm_116

Phlegm_117

Phlegm_118

Phlegm_119

Phlegm_120

Phlegm_121

Phlegm_122

Phlegm_123

Phlegm_124

Phlegm_125

Phlegm_126

Phlegm_127

Phlegm_128

Phlegm_129

Phlegm_130
(tail sheath protein)

Phlegm_132

Phlegm_133

Phlegm_134
(tail assembly chaperone)

Phlegm_135
(tail assembly chaperone)

Phlegm_136
(tape measure protein)

Phlegm_137

Phlegm_138

Phlegm_139

Phlegm_140
(minor tail protein)

Phlegm_141

Phlegm_142
(minor tail protein)

Phlegm_143
(baseplate wedge protein)

Phlegm_144
(baseplate J protein)

Phlegm_145
(HNH endonuclease)

Phlegm_146

Phlegm_147

Phlegm_148

Phlegm_149
(minor tail protein)

Phlegm_150

Phlegm_151

Phlegm_152

Phlegm_153

Phlegm_154

Phlegm_155

Phlegm_156

Phlegm_157

Phlegm_161

Phlegm_162
(peptidyl tRNA hydrolase)

Phlegm_176
(HNH endonuclease)

Phlegm_178
(minor tail protein)

Phlegm_179

Phlegm_190

Phlegm_191
(DNA helicase)

Phlegm_192

Phlegm_193

Phlegm_194

Phlegm_195

Phlegm_196

Phlegm_197

Phlegm_198

Phlegm_199

Phlegm_200

Phlegm_201
(thymidylate kinase)

Phlegm_202
(aminotransferase)

Phlegm_203

Phlegm_204

Phlegm_205

Phlegm_206
(DnaC-like helicase loader)

Phlegm_207
(DnaB-like dsDNA helicase)

Phlegm_208

Phlegm_209
(DNA primase)

Phlegm_210

Phlegm_211
(DnaJ-like chaperonin)

Phlegm_212
(ssDNA binding protein)

Phlegm_213
(DNA polymerase III alpha)

Phlegm_214
(RF-1 peptide chain release factor)

Phlegm_215
(DNA recombinase)

Phlegm_216
(holliday junction resolvase)

Phlegm_217

Phlegm_218
(HNH endonuclease)

Phlegm_219
(resolvase)

Phlegm_220

Phlegm_221

Phlegm_222

Phlegm_223

Phlegm_224

Phlegm_225

Phlegm_226

Phlegm_229

Phlegm_230

Phlegm_231

Phlegm_232

Phlegm_233

Phlegm_234

Phlegm_235

Phlegm_236

Phlegm_237
(Ro-like RNA binding protein)

Phlegm_238

Phlegm_239

Phlegm_240

Phlegm_241

Phlegm_242

Phlegm_243

Phlegm_244

Phlegm_245
(glycosyltransferase)

Phlegm_246

Phlegm_247
(glycosyltransferase)

Phlegm_248
(galactosyltransferase)

Phlegm_249

Phlegm_250
(glycosyltransferase)

Phlegm_251

Phlegm_252

Phlegm_253

Phlegm_254

Phlegm_255
(lysin A)

Phlegm_256
(holin)

Phlegm_257
(lysin B)

Phlegm_258
(terminase)

Phlegm_259

Phlegm_260

Phlegm_261

Phlegm_262

Phlegm_263
(polynucleotide kinase)

Phlegm_264

Phlegm_265
(serine/threonine kinase)

Phlegm_266

Phlegm_267

Phlegm_268
(PurA-like adenylosuccinate synthetase)

Phlegm_269

Phlegm_270

Phlegm_271

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH697590
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 61
Pitt Freezer Box Grid# B8
Available Files
GenBank File for PhameratorDownload