Mycobacterium phage Roots515
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Detailed Information for Phage Roots515
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCourtney Mithelman
Year Found2017
Location FoundJohnston, IA United States
Finding InstitutionNorthwestern College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates41.6597 N, 93.7207 W Map
Discovery NotesSoil sample was wet and taken from slightly below surface level from a garden. Current outside temperature at time of selecting sample was 48 degrees Fahrenheit and raining.
Naming NotesSample was found in the Des Moines area, which has an area code of 515. Roots was used because Des Moines is my hometown.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)156288
Character of genome endsUnknown
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

Roots515 (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesPlaque was clear throughout purification steps. Size of plaque did change quite a bit at different purification points, varying from very large to very small. However, steps were taken to ensure that only one virus was present.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload