Mycobacterium phage Roots515
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Detailed Information for Phage Roots515
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCourtney Mithelman
Year Found2017
Location FoundJohnston, IA United States
Finding InstitutionNorthwestern College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates41.6597 N, 93.7207 W Map
Discovery NotesSoil sample was wet and taken from slightly below surface level from a garden. Current outside temperature at time of selecting sample was 48 degrees Fahrenheit and raining.
Naming NotesSample was found in the Des Moines area, which has an area code of 515. Roots was used because Des Moines is my hometown.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage931
Genome length (bp)156288
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesPlaque was clear throughout purification steps. Size of plaque did change quite a bit at different purification points, varying from very large to very small. However, steps were taken to ensure that only one virus was present.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Roots515_1

Roots515_2

Roots515_3
(Nucleotidyl transferase)

Roots515_4

Roots515_5

Roots515_6

Roots515_7

Roots515_8

Roots515_9

Roots515_10

Roots515_11

Roots515_12

Roots515_13

Roots515_14

Roots515_15

Roots515_16

Roots515_17

Roots515_18
(Histidine triad nucleotide binding protein)

Roots515_19

Roots515_20

Roots515_21

Roots515_22

Roots515_23

Roots515_24

Roots515_25

Roots515_26

Roots515_27

Roots515_28

Roots515_29
(Membrane protein, Band-7-like)

Roots515_30

Roots515_31

Roots515_32
(helix-turn-helix DNA binding domain)

Roots515_33

Roots515_34

Roots515_35

Roots515_36

Roots515_37

Roots515_38

Roots515_39

Roots515_40

Roots515_41

Roots515_42

Roots515_43

Roots515_44

Roots515_45

Roots515_46

Roots515_47

Roots515_48

Roots515_49

Roots515_50

Roots515_51

Roots515_52

Roots515_53

Roots515_54

Roots515_55

Roots515_56

Roots515_57

Roots515_58

Roots515_59

Roots515_60

Roots515_61

Roots515_62
(helix-turn-helix DNA binding domain)

Roots515_63

Roots515_64

Roots515_65

Roots515_66

Roots515_67

Roots515_68

Roots515_69

Roots515_70

Roots515_71

Roots515_72

Roots515_73

Roots515_74

Roots515_75

Roots515_76

Roots515_77

Roots515_78

Roots515_79

Roots515_80

Roots515_81

Roots515_82

Roots515_87

Roots515_88

Roots515_89
(portal protein)

Roots515_90

Roots515_91

Roots515_92
(ThyX thymidylate synthase)

Roots515_93

Roots515_94
(phosphoesterase)

Roots515_95
(endolysin, LysM-like)

Roots515_96

Roots515_97

Roots515_98
(Major capsid protein)

Roots515_99

Roots515_100

Roots515_101

Roots515_102

Roots515_103

Roots515_104

Roots515_105

Roots515_106

Roots515_107

Roots515_108

Roots515_109

Roots515_110

Roots515_111

Roots515_112

Roots515_113
(Capsid decoration protein)

Roots515_114

Roots515_115

Roots515_116

Roots515_117

Roots515_118

Roots515_119

Roots515_120

Roots515_121

Roots515_122

Roots515_123

Roots515_124

Roots515_125

Roots515_126
(Tail sheath protein)

Roots515_127

Roots515_128

Roots515_129
(Tail assembly chaperone)

Roots515_130
(Tail assembly chaperone)

Roots515_131
(tape measure protein)

Roots515_132

Roots515_133

Roots515_134

Roots515_135
(Minor tail protein)

Roots515_136

Roots515_137
(Minor tail protein)

Roots515_138
(baseplate J protein)

Roots515_139
(baseplate J protein)

Roots515_140
(HNH endonuclease)

Roots515_141

Roots515_142

Roots515_143

Roots515_144
(Minor tail protein)

Roots515_145

Roots515_146

Roots515_147

Roots515_148

Roots515_149

Roots515_150

Roots515_151

Roots515_152

Roots515_153

Roots515_154

Roots515_158

Roots515_159
(peptidyl tRNA hydrolase)

Roots515_166

Roots515_172

Roots515_175
(HNH endonuclease)

Roots515_177
(Minor tail protein, D-ala-D-ala carboxypeptidase)

Roots515_178

Roots515_189

Roots515_190
(DNA helicase)

Roots515_191

Roots515_192

Roots515_193

Roots515_194

Roots515_195

Roots515_196

Roots515_197

Roots515_198

Roots515_199

Roots515_200

Roots515_201
(Aminotransferase)

Roots515_202

Roots515_203

Roots515_204
(DnaC helicase loader)

Roots515_205
(DnaB dsDNA helicase)

Roots515_206

Roots515_207
(DNA primase)

Roots515_208

Roots515_209
(chaperonin, DNA -J-like)

Roots515_210
(ssDNA binding protein)

Roots515_211
(DnaE DNA polymerase III (alpha))

Roots515_212
(RF-1 peptide chain release factor)

Roots515_213
(RecA DNA recombinase)

Roots515_214

Roots515_215

Roots515_216
(HNH endonuclease)

Roots515_217
(RusA resolvase (endonuclease))

Roots515_218

Roots515_219

Roots515_220

Roots515_221

Roots515_222

Roots515_223

Roots515_224

Roots515_227

Roots515_228

Roots515_229

Roots515_230

Roots515_231

Roots515_232

Roots515_233

Roots515_234

Roots515_235

Roots515_236
(Ro RNA binding protein)

Roots515_237

Roots515_238

Roots515_239

Roots515_240

Roots515_241

Roots515_242

Roots515_243
(Glycosyltransferase)

Roots515_244

Roots515_245
(Galactosyltransferase)

Roots515_246
(Galactosyltransferase)

Roots515_247
(nicotinate ribosyltransferase)

Roots515_248
(Glycosyltransferase)

Roots515_249
(capsid decoration protein)

Roots515_250

Roots515_251

Roots515_252

Roots515_253
(lysin A)

Roots515_254
(Holin)

Roots515_255
(lysin B)

Roots515_256
(terminase)

Roots515_257

Roots515_258

Roots515_259

Roots515_260

Roots515_261
(polynucleotide kinase)

Roots515_262

Roots515_263

Roots515_264
(serine/threonine kinase)

Roots515_265

Roots515_266

Roots515_267
(adenylsuccinate synthetase, PurA like)

Roots515_268

Roots515_269

Roots515_270

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH744422
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 72
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload