Mycobacterium phage Wally
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Detailed Information for Phage Wally
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRachel Nichole Walstead
Year Found2010
Location FoundMechanicsville, VA USA
Finding InstitutionVirginia Commonwealth University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates37.616 N, 77.363475 W Map
Discovery NotesCollected from soil under mulch (~3") in a flower bed
Approximately 80°F weather in late August
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2011
Genome length (bp)155299
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques range from 0.5mm - 2mm in diameter, all plates show large and small plaques. All plaques are clear
MorphotypeMyoviridae
Number of Genes228
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Wally_1

Wally_2

Wally_3
(nucleotidyl transferase)

Wally_4

Wally_5

Wally_6

Wally_7

Wally_8

Wally_9

Wally_10

Wally_11

Wally_12

Wally_13

Wally_14

Wally_15

Wally_16

Wally_17
(histidine triad nucleotide binding protein)

Wally_18

Wally_19

Wally_20

Wally_21

Wally_22

Wally_23

Wally_24

Wally_25

Wally_26

Wally_27

Wally_28
(band-7-like membrane protein)

Wally_29

Wally_30

Wally_31

Wally_32

Wally_33

Wally_34

Wally_35

Wally_36

Wally_37

Wally_38

Wally_39

Wally_40

Wally_41

Wally_42

Wally_43

Wally_44

Wally_45

Wally_46

Wally_47

Wally_48

Wally_49

Wally_50

Wally_51

Wally_52

Wally_53

Wally_54

Wally_55

Wally_56

Wally_57

Wally_58

Wally_59

Wally_60

Wally_61
(helix-turn-helix DNA binding domain protein)

Wally_62

Wally_63

Wally_64

Wally_65

Wally_66

Wally_67

Wally_68

Wally_69

Wally_70

Wally_71

Wally_72

Wally_73

Wally_74

Wally_75

Wally_76

Wally_77

Wally_78

Wally_79

Wally_80

Wally_81

Wally_82 — tRNA
(gp82; tRNA-Ser(gct))

Wally_83 — tRNA
(gp83; tRNA-ser(cga))

Wally_84 — tRNA
(gp84; tRNA-Leu(cag))

Wally_85 — tRNA
(gp85; tRNA-Leu(gag))

Wally_86 — tRNA
(gp86; tRNA-Leu(caa))

Wally_87

Wally_88

Wally_89
(portal protein)

Wally_90

Wally_91

Wally_92
(ThyX-like thymidylate synthase)

Wally_93

Wally_94
(RNA ligase)

Wally_95

Wally_96

Wally_97

Wally_98
(major capsid protein)

Wally_99

Wally_100

Wally_101

Wally_102

Wally_103

Wally_104

Wally_105

Wally_106

Wally_107

Wally_108

Wally_109

Wally_110

Wally_111

Wally_112

Wally_113

Wally_114

Wally_115

Wally_116

Wally_117

Wally_118

Wally_119

Wally_120

Wally_121

Wally_122

Wally_123

Wally_124

Wally_125

Wally_126
(tail sheath protein)

Wally_127

Wally_128

Wally_129
(tail assembly chaperone)

Wally_130
(tail assembly chaperone)

Wally_131
(tape measure protein)

Wally_132

Wally_133

Wally_134

Wally_135
(minor tail protein)

Wally_136

Wally_137
(minor tail protein)

Wally_138

Wally_139
(baseplate J protein)

Wally_140

Wally_141

Wally_142

Wally_143

Wally_144
(minor tail protein)

Wally_145

Wally_146

Wally_147

Wally_148

Wally_149

Wally_150

Wally_151

Wally_152 — tRNA
(gp152; tRNA-Pro(tgg))

Wally_153 — tRNA
(gp153; tRNA-Trp(cca))

Wally_154 — tRNA
(gp154; tRNA-Tyr(gta))

Wally_155

Wally_156
(peptidyl tRNA hydrolase)

Wally_157 — tRNA
(gp157; tRNA-Other(cta))

Wally_158 — tRNA
(gp158; tRNA-Met(cat))

Wally_159 — tRNA
(gp159; tRNA-Cys(gca))

Wally_160 — tRNA
(gp160; tRNA-Glu(ctc))

Wally_161 — tRNA
(gp161; tRNA-His(gtg))

Wally_162

Wally_163 — tRNA
(gp163; tRNA-Ala(tgc))

Wally_164 — tRNA
(gp164; tRNA-Phe(gaa))

Wally_165 — tRNA
(gp165; tRNA-Val(cac))

Wally_166 — tRNA
(gp166; tRNA-Lys(ctt))

Wally_167 — tRNA
(gp167; tRNA-Glu(ttc))

Wally_168 — tRNA
(gp168; tRNA-Gly(tcc))

Wally_169 — tRNA
(gp169; tRNA-Thr(cgt))

Wally_170 — tRNA
(gp170; tRNA-Thr(tgt))

Wally_171

Wally_172 — tRNA
(gp172; tRNA-Thr(ggt))

Wally_173
(minor tail protein)

Wally_174

Wally_175 — tRNA
(gp175; tRNA-Gly(gcc))

Wally_176 — tRNA
(gp176; tRNA-Asp(gtc))

Wally_177 — tRNA
(gp177; tRNA-Met(cat))

Wally_178 — tRNA
(gp178; tRNA-Ile(gat))

Wally_179 — tRNA
(gp179; tRNA-Arg(acg))

Wally_180 — tRNA
(gp180; tRNA-Val(gac))

Wally_181 — tRNA
(gp181; tRNA-Arg(cct))

Wally_182 — tmRNA
(tmRNA)

Wally_183 — tRNA
(gp183; tRNA-Gln(ttg))

Wally_184 — tRNA
(gp184; tRNA-Arg(tct))

Wally_185 — tRNA
(gp185; tRNA-Lys(ttt))

Wally_186

Wally_187

Wally_188
(DNA helicase)

Wally_189

Wally_190

Wally_191

Wally_192

Wally_193

Wally_194

Wally_195

Wally_196

Wally_197
(aminotransferase)

Wally_198

Wally_199

Wally_200
(DnaC-like helicase loader)

Wally_201
(DnaB-like dsDNA helicase)

Wally_202

Wally_203
(DNA primase)

Wally_204

Wally_205
(DnaJ-like chaperonin)

Wally_206

Wally_207
(DnaE-like DNA polymerase III)

Wally_208
(RF-1 peptide chain release factor)

Wally_209
(RecA-like DNA recombinase)

Wally_210

Wally_211

Wally_212
(HNH endonuclease)

Wally_213
(RusA-like resolvase)

Wally_214

Wally_215

Wally_216

Wally_217

Wally_218

Wally_219

Wally_220

Wally_221 — tRNA
(gp221; tRNA-Gln(ctg))

Wally_222 — tRNA
(gp222; tRNA-Asn(gtt))

Wally_223

Wally_224

Wally_225

Wally_226

Wally_227

Wally_228

Wally_229

Wally_230

Wally_231
(Ro-like RNA binding protein)

Wally_232

Wally_233

Wally_234

Wally_235

Wally_236

Wally_237

Wally_238
(glycosyltransferase)

Wally_239

Wally_240
(glycosyltransferase)

Wally_241
(glycosyltransferase)

Wally_242
(PnuC-like nicotinamide riboside transporter)

Wally_243
(glycosyltransferase)

Wally_244

Wally_245

Wally_246

Wally_247

Wally_248
(lysin A)

Wally_249
(holin)

Wally_250
(lysin B)

Wally_251
(terminase)

Wally_252

Wally_253

Wally_254

Wally_255

Wally_256
(polynucleotide kinase)

Wally_257

Wally_258
(serine/threonine kinase)

Wally_259

Wally_260

Wally_261
(PurA-like adenylosuccinate synthetase)

Wally_262

Wally_263

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN699625
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2010VACUwallyRNW
SEA Lysate Titer 6.0e10
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload