Mycobacterium phage Dalmuri
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Detailed Information for Phage Dalmuri
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJenna Rowlett
Year Found2015
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.984987 N, 86.457712 W Map
Discovery NotesThe soil I found it in originally was dark brown and crumbly, it was pretty far from any kind of water source. The sample site was in a wooded area right outside of the Craven's library on WKU's campus and the day I collected the soil it was about 87 degrees Fahrenheit and I dug about 2.5 cm to get to the "good" dirt.
Naming NotesI decided to name my phage Dalmuri, which in Korean for "halo around the moon." This I felt was analogous to how my plaque phenotype looks, because the plaques have a "ring" morphology.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 30, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage193
Genome length (bp)59708
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesClear plaque mutants began to pop up over time.
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesMy plaques have two distinct phenotypes, one is approximately 1mm in diameter and the other is 4mm in diameter(this makes up the majority of the plaques). Also along the way there were clear plaque mutants that popped up in the population.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Dalmuri_1

Dalmuri_2

Dalmuri_3

Dalmuri_4

Dalmuri_6

Dalmuri_7
(terminase small subunit)

Dalmuri_8
(terminase large subunit)

Dalmuri_9
(portal protein)

Dalmuri_10
(capsid maturation protease)

Dalmuri_11

Dalmuri_12
(scaffolding protein)

Dalmuri_13
(major capsid protein)

Dalmuri_14
(head-to-tail connector complex protein)

Dalmuri_15
(head-to-tail connector complex protein)

Dalmuri_16
(head-to-tail connector complex protein)

Dalmuri_17
(head-to-tail connector complex protein)

Dalmuri_18
(major tail protein)

Dalmuri_19
(tail assembly chaperone)

Dalmuri_20
(tail assembly chaperone)

Dalmuri_21
(tape measure protein)

Dalmuri_22
(minor tail protein)

Dalmuri_23
(minor tail protein)

Dalmuri_24
(minor tail protein)

Dalmuri_25
(minor tail protein)

Dalmuri_26
(minor tail protein)

Dalmuri_27
(minor tail protein)

Dalmuri_28
(minor tail protein)

Dalmuri_29

Dalmuri_30
(lysin A)

Dalmuri_31
(lysin B)

Dalmuri_32
(holin)

Dalmuri_33

Dalmuri_34

Dalmuri_35
(exonuclease)

Dalmuri_36
(helix-turn-helix DNA binding domain protein)

Dalmuri_37

Dalmuri_38

Dalmuri_39

Dalmuri_40

Dalmuri_41
(integrase)

Dalmuri_42
(membrane protein)

Dalmuri_43
(immunity repressor)

Dalmuri_44
(Cro protein)

Dalmuri_45
(excise)

Dalmuri_46

Dalmuri_47
(membrane protein)

Dalmuri_48

Dalmuri_49
(helix-turn-helix DNA binding domain protein)

Dalmuri_50
(WhiB family transcription factor)

Dalmuri_51

Dalmuri_52

Dalmuri_53

Dalmuri_54

Dalmuri_55

Dalmuri_56

Dalmuri_57
(DNA polymerase III subunit)

Dalmuri_58

Dalmuri_59

Dalmuri_60

Dalmuri_61
(exonuclease)

Dalmuri_62

Dalmuri_63

Dalmuri_64

Dalmuri_65

Dalmuri_66
(NrdH-like glutaredoxin)

Dalmuri_67
(membrane protein)

Dalmuri_68
(HNH endonuclease)

Dalmuri_69
(DNA primase/polymerase)

Dalmuri_70

Dalmuri_71
(RusA-like resolvase (endonuclease))

Dalmuri_72

Dalmuri_73
(helix-turn-helix DNA binding domain protein)

Dalmuri_74

Dalmuri_75

Dalmuri_76
(SprT-like protease)

Dalmuri_77

Dalmuri_78

Dalmuri_79
(membrane protein)

Dalmuri_80

Dalmuri_81

Dalmuri_82

Dalmuri_83

Dalmuri_84

Dalmuri_85
(rtcB-like RNA ligase)

Dalmuri_86

Dalmuri_87

Dalmuri_88

Dalmuri_89

Dalmuri_90

Dalmuri_91
(HicB-like antitoxin)

Dalmuri_92
(HicA-like toxin)

Dalmuri_93

Dalmuri_94

Dalmuri_95

Dalmuri_96

Dalmuri_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH727544
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2015WEKUDalmuriJRR
SEA Lysate Titer 1.64x10^9
Date of SEA Lysate Titering Oct 27, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload