Mycobacterium phage BEEST
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Detailed Information for Phage BEEST
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLisa Deng and Patrick Ng
Year Found2008
Location FoundSt Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.650249 N, 90.316691 W Map
Discovery NotesThe soil was collected from a site that is used as a municipal mulch pile. Soil depth 1cm at 70 degrees.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 31, 2011
Genome length (bp)59906
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque Notes3mm/turbid and haloed/ incubation 24 hours at 37 degrees Celsius
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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BEEST_1

BEEST_2

BEEST_3

BEEST_4

BEEST_6

BEEST_7
(terminase small subunit)

BEEST_8
(terminase large subunit)

BEEST_9
(portal protein)

BEEST_10
(capsid maturation protease)

BEEST_11

BEEST_12
(scaffolding protein)

BEEST_13
(major capsid protein)

BEEST_14
(head-to-tail connector complex protein)

BEEST_15
(head-to-tail connector complex protein)

BEEST_16

BEEST_17
(head-to-tail connector complex protein)

BEEST_18
(major tail protein)

BEEST_19
(tail assembly chaperone)

BEEST_20
(tail assembly chaperone)

BEEST_21
(tape measure protein)

BEEST_22
(minor tail protein)

BEEST_23
(minor tail protein)

BEEST_24
(minor tail protein)

BEEST_25
(minor tail protein)

BEEST_26
(minor tail protein)

BEEST_27
(minor tail protein)

BEEST_28
(minor tail protein)

BEEST_29

BEEST_30
(lysin A)

BEEST_31
(lysin B)

BEEST_32
(holin)

BEEST_33

BEEST_34

BEEST_35
(exonuclease)

BEEST_36
(helix-turn-helix DNA binding domain protein)

BEEST_37

BEEST_38

BEEST_39

BEEST_40

BEEST_41
(integrase)

BEEST_42

BEEST_43
(immunity repressor)

BEEST_44
(helix-turn-helix DNA binding domain protein)

BEEST_45
(excise)

BEEST_46

BEEST_47

BEEST_48

BEEST_49
(MerR-like HTH DNA binding protein)

BEEST_50
(WhiB family transcription factor)

BEEST_51

BEEST_52

BEEST_53

BEEST_54

BEEST_55

BEEST_56

BEEST_57
(DNA polymerase III subunit)

BEEST_58

BEEST_59

BEEST_60

BEEST_61
(exonuclease)

BEEST_62

BEEST_63

BEEST_64

BEEST_65

BEEST_66
(NrdH-like glutaredoxin)

BEEST_67

BEEST_68
(HNH endonuclease)

BEEST_69
(DNA primase)

BEEST_70

BEEST_71
(RusA-like resolvase)

BEEST_72

BEEST_73
(helix-turn-helix DNA binding domain protein)

BEEST_74

BEEST_75

BEEST_76
(SprT-like protease)

BEEST_77

BEEST_78

BEEST_79
(helix-turn-helix DNA binding domain protein)

BEEST_80

BEEST_81
(helix-turn-helix DNA binding domain protein)

BEEST_82

BEEST_83

BEEST_84

BEEST_85
(RtcB-like RNA ligase)

BEEST_86

BEEST_87

BEEST_88

BEEST_89

BEEST_90

BEEST_91
(HicB-like antitoxin)

BEEST_92
(HicA-like toxin)

BEEST_93

BEEST_94

BEEST_95

BEEST_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH509444
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.2e10
Pitt Freezer Box# 10
Pitt Freezer Box Grid# 9
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload