Mycobacterium phage AlleyCat
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Detailed Information for Phage AlleyCat
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesMolesto
Found ByJodi Elaine Thomas
Year Found2015
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.5982 N, 86.26556 W Map
Discovery NotesThrough working with this phage I discovered that this phage was a huge nuisance. The purification process took 6 rounds to get 3 pure plaque plates. During the purification process we found that the plaque had a clear plaque mutant, which was the reason for the confusion during the purification process. Even though the phage was was difficult, it did lead to interesting results. The gel taken was very fascinating, and the EM pictures were interesting as well.
Naming NotesI named my phage AlleyCat, because I collected the dirt sample that I found the phage in in an alley.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2914
Genome length (bp)62112
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kahlo (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesThe plaques were 2.5 mm and bulls-eye.
MorphotypeSiphoviridae
Final annotation complete?Yes
Has been Phamerated?Yes
Gene List

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AlleyCat_1

AlleyCat_2

AlleyCat_3

AlleyCat_5
(terminase small subunit)

AlleyCat_6
(terminase large subunit)

AlleyCat_7
(portal protein)

AlleyCat_8
(capsid maturation protease)

AlleyCat_9

AlleyCat_10
(scaffolding protein)

AlleyCat_11
(major capsid protein)

AlleyCat_12
(head-to-tail connector protein)

AlleyCat_13
(head-to-tail connector protein)

AlleyCat_14
(head-to-tail connector protein)

AlleyCat_15
(head-to-tail connector protein)

AlleyCat_16
(major tail subunit)

AlleyCat_17
(tail assembly chaperone)

AlleyCat_18
(tail assembly chaperone)

AlleyCat_19
(tape measure protein)

AlleyCat_20
(minor tail protein)

AlleyCat_21
(minor tail protein)

AlleyCat_22
(minor tail protein)

AlleyCat_23
(minor tail protein)

AlleyCat_24
(minor tail protein)

AlleyCat_25
(minor tail protein)

AlleyCat_26
(minor tail protein)

AlleyCat_27

AlleyCat_28
(minor tail protein)

AlleyCat_29

AlleyCat_30
(lysin A)

AlleyCat_31
(lysin B)

AlleyCat_32
(holin)

AlleyCat_33

AlleyCat_34

AlleyCat_35

AlleyCat_36

AlleyCat_37
(HTH DNA binding protein)

AlleyCat_38
(hydrolase)

AlleyCat_39
(integrase)

AlleyCat_40

AlleyCat_41

AlleyCat_42
(immunity repressor)

AlleyCat_43
(CRO protein)

AlleyCat_44

AlleyCat_45

AlleyCat_46

AlleyCat_47

AlleyCat_48

AlleyCat_49

AlleyCat_50

AlleyCat_51

AlleyCat_52

AlleyCat_53

AlleyCat_54

AlleyCat_55

AlleyCat_56

AlleyCat_57
(exonuclease)

AlleyCat_58

AlleyCat_59

AlleyCat_60

AlleyCat_61

AlleyCat_62
(glutaredoxin)

AlleyCat_63
(HNH endonuclease)

AlleyCat_64
(HNH endonuclease)

AlleyCat_65
(DNA primase/polymerase)

AlleyCat_66
(DNA helicase)

AlleyCat_67
(resolvase)

AlleyCat_68
(peptidase)

AlleyCat_69

AlleyCat_70

AlleyCat_71

AlleyCat_72

AlleyCat_73

AlleyCat_74

AlleyCat_75

AlleyCat_76

AlleyCat_77

AlleyCat_78
(RNA ligase)

AlleyCat_79

AlleyCat_80

AlleyCat_81

AlleyCat_82

AlleyCat_83

AlleyCat_84

AlleyCat_85

AlleyCat_86

AlleyCat_87
(queuine tRNA-ribosyltransferase)

AlleyCat_88

AlleyCat_89

AlleyCat_90

AlleyCat_91

AlleyCat_92

AlleyCat_93

AlleyCat_94

AlleyCat_95

AlleyCat_96

AlleyCat_97

AlleyCat_98

AlleyCat_99

AlleyCat_100
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF185717
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload