Mycobacterium phage LastHope
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Detailed Information for Phage LastHope
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLysangelie I. Gonzalez-Santos
Year Found2015
Location FoundJuana Diaz, PR USA
Finding InstitutionUniversity of Puerto Rico at Cayey
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates17.995028 N, 66.516494 W Map
Discovery NotesSample came from a crop field. The soil was dry.
Naming NotesI had made many attempts without success and was losing hope until I finally found LastHope.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage854
Genome length (bp)60934
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesClear plaques, approximately 1.5 mm in diameter.
MorphotypeSiphoviridae
Final annotation complete?Yes
Has been Phamerated?Yes
Gene List

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LastHope_1

LastHope_2

LastHope_3

LastHope_4

LastHope_6

LastHope_7
(terminase small subunit)

LastHope_8
(terminase large subunit)

LastHope_9
(portal protein)

LastHope_10
(capsid maturation protease)

LastHope_11

LastHope_12
(scaffolding protein)

LastHope_13
(major capsid protein)

LastHope_14
(head-to-tail connector protein)

LastHope_15
(head-to-tail connector protein)

LastHope_16
(head-to-tail connector protein)

LastHope_17
(head-to-tail connector protein)

LastHope_18
(major tail protein)

LastHope_19
(tail assembly chaperone)

LastHope_20
(tail assembly chaperone)

LastHope_21
(tape measure protein)

LastHope_22
(minor tail protein)

LastHope_23
(minor tail protein)

LastHope_24
(minor tail protein)

LastHope_25
(minor tail protein)

LastHope_26
(minor tail protein)

LastHope_27
(minor tail protein)

LastHope_28
(minor tail protein)

LastHope_29

LastHope_30
(lysin A)

LastHope_31
(lysin B)

LastHope_32
(holin)

LastHope_33

LastHope_34

LastHope_35
(exonuclease)

LastHope_36

LastHope_37

LastHope_38

LastHope_39

LastHope_40

LastHope_41

LastHope_42

LastHope_43
(integrase)

LastHope_44

LastHope_45

LastHope_46
(immunity repressor)

LastHope_47

LastHope_48

LastHope_49

LastHope_50

LastHope_51

LastHope_52
(WhiB family transcription factor)

LastHope_53

LastHope_54

LastHope_55

LastHope_56

LastHope_57

LastHope_58

LastHope_59

LastHope_60
(DNA polymerase III subunit)

LastHope_61

LastHope_62

LastHope_63

LastHope_64
(exonuclease)

LastHope_65

LastHope_66

LastHope_67

LastHope_68

LastHope_69

LastHope_70

LastHope_71

LastHope_72

LastHope_73
(DNA primase)

LastHope_74

LastHope_75
(resolvase)

LastHope_76

LastHope_77

LastHope_78

LastHope_79
(peptidase)

LastHope_80

LastHope_81

LastHope_82
(HTH DNA binding protein)

LastHope_83
(protease)

LastHope_84

LastHope_85

LastHope_86

LastHope_87

LastHope_88

LastHope_89

LastHope_90

LastHope_91
(RNA ligase)

LastHope_92

LastHope_93

LastHope_94

LastHope_95
(HTH DNA binding protein)

LastHope_96

LastHope_97
(HTH DNA binding protein)

LastHope_98

LastHope_99

LastHope_100

LastHope_101

LastHope_102

LastHope_103
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140416
Refseq NumberNone yet
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload