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Mycobacterium phage LastHope
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Detailed Information for Phage LastHope
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLysangelie I. Gonzalez-Santos
Year Found2015
Location FoundJuana Diaz, PR USA
Finding InstitutionUniversity of Puerto Rico at Cayey
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates17.995028 N, 66.516494 W Map
Discovery NotesSample came from a crop field. The soil was dry.
Naming NotesI had made many attempts without success and was losing hope until I finally found LastHope.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage854
Genome length (bp)60934
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Other Cluster Members

Click to View

Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesClear plaques, approximately 1.5 mm in diameter.
MorphotypeSiphoviridae
Final annotation complete?Yes
Has been Phamerated?Yes
Gene List

Click to View

LastHope_1

LastHope_2

LastHope_3

LastHope_4

LastHope_6
(terminase, small subunit)

LastHope_7
(terminase, large subunit)

LastHope_8
(portal protein)

LastHope_9
(portal protein)

LastHope_10
(capsid maturation protease)

LastHope_11

LastHope_12
(scaffolding protein)

LastHope_13
(major capsid protein)

LastHope_14
(head-to-tail connector protein)

LastHope_15
(head-to-tail connector protein)

LastHope_16
(head-to-tail connector protein)

LastHope_17
(head-to-tail connector protein)

LastHope_18
(major tail protein)

LastHope_19
(tail assembly chaperone)

LastHope_20
(tail assembly chaperone)

LastHope_21
(tape measure protein)

LastHope_22
(minor tail protein)

LastHope_23
(minor tail protein)

LastHope_24
(minor tail protein)

LastHope_25
(minor tail protein)

LastHope_26
(minor tail protein)

LastHope_27
(minor tail protein)

LastHope_28
(minor tail protein)

LastHope_29

LastHope_30
(lysin A)

LastHope_31
(lysin B)

LastHope_32
(holin)

LastHope_33
(membrane protein)

LastHope_34

LastHope_35
(MRE11 double-strand break endo/exonuclease)

LastHope_36
(membrane protein)

LastHope_37

LastHope_38
(helix-turn-helix DNA binding domain)

LastHope_39

LastHope_40

LastHope_41
(membrane protein)

LastHope_42
(integrase, (S-int))

LastHope_43

LastHope_44
(membrane protein)

LastHope_45
(immunity repressor)

LastHope_46
(CRO (control of repressor's operator))

LastHope_47

LastHope_48
(membrane protein)

LastHope_49

LastHope_50
(helix-turn-helix DNA binding domain)

LastHope_51
(WhiB family transcription factor)

LastHope_52

LastHope_53

LastHope_54

LastHope_55

LastHope_56

LastHope_57

LastHope_58
(DnaQ exonuclease (DNA polymerase III subunit))

LastHope_59

LastHope_60

LastHope_61

LastHope_62
(exonuclease)

LastHope_63

LastHope_64

LastHope_65

LastHope_66

LastHope_67

LastHope_68

LastHope_69
(NrdH glutaredoxin)

LastHope_70
(HNH endonuclease)

LastHope_71

LastHope_72

LastHope_73
(RusA resolvase (endonuclease))

LastHope_74

LastHope_75

LastHope_76

LastHope_77
(HTH DNA binding protein)

LastHope_78

LastHope_79

LastHope_80
(esterase)

LastHope_81
(SprT protease)

LastHope_82

LastHope_83

LastHope_84
(HTH DNA binding protein)

LastHope_85

LastHope_86
(HTH DNA binding protein)

LastHope_87

LastHope_88
(polynucleotitde kinase)

LastHope_89
(rtcB RNA ligase)

LastHope_90

LastHope_91

LastHope_92

LastHope_93
(DNA binding protein)

LastHope_94

LastHope_95
(antitoxin in toxin/antitoxin system, HicB-like)

LastHope_96
(toxin in toxin/antitoxin system, HicA-like)

LastHope_97
(membrane protein)

LastHope_98

LastHope_99

LastHope_100

LastHope_101
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140416
Refseq NumberNone yet
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload