Mycobacterium phage Waterfoul
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Detailed Information for Phage Waterfoul
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElla Embry and Paige Jackson
Year Found2015
Location FoundHattiesburg, MS United States
Finding InstitutionUniversity of Southern Mississippi
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates31.323056 N, 89.316389 W Map
Discovery NotesFound on the shoreline of a man-made lake at the Hattiesburg Zoo. It came from wet soil underneath the feet of some geese. It was taken at surface level, and the weather was a sunny summer day around 79º Fahrenheit
Naming NotesWe chose the name Waterfoul because our soil sample came from under neither some ornery geese we had to shoo away in order to get at the soil. Water fowl are birds that live and thrive near water, the word foul means ill-tempered. Thus, we used the pun Waterfoul to name our phage and make a specific nod to where it was found.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3046
Genome length (bp)61248
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content64.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesOur phage undergoes a lytic cycle and produces large, clear plaques. They are around 3mm in diameter.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Waterfoul_1

Waterfoul_2

Waterfoul_3

Waterfoul_5
(Terminase, small subunit)

Waterfoul_6
(Terminase, large subunit)

Waterfoul_7
(Portal)

Waterfoul_8
(Capsid maturation protease)

Waterfoul_9

Waterfoul_10
(Scaffolding protein)

Waterfoul_11
(Major capsid)

Waterfoul_12
(Head-to-tail connector)

Waterfoul_13
(Head-to-tail connector)

Waterfoul_14
(Head-to-tail connector)

Waterfoul_15
(Head-to-tail connector)

Waterfoul_16
(Major tail)

Waterfoul_17
(Tail assembly chaperone)

Waterfoul_18
(Tail assembly chaperone)

Waterfoul_19
(Tape measure)

Waterfoul_20
(Minor tail)

Waterfoul_21
(Minor tail)

Waterfoul_22
(Minor tail)

Waterfoul_23
(Minor tail)

Waterfoul_24

Waterfoul_25
(Minor tail)

Waterfoul_26

Waterfoul_27

Waterfoul_28

Waterfoul_29
(Lysin A)

Waterfoul_30
(Lysin B)

Waterfoul_31
(Holin)

Waterfoul_32

Waterfoul_33

Waterfoul_34

Waterfoul_35

Waterfoul_36

Waterfoul_37

Waterfoul_38

Waterfoul_39
(Integrase)

Waterfoul_40

Waterfoul_41

Waterfoul_42
(Immunity repressor)

Waterfoul_43

Waterfoul_44

Waterfoul_45

Waterfoul_46

Waterfoul_47

Waterfoul_48

Waterfoul_49

Waterfoul_50

Waterfoul_51

Waterfoul_52

Waterfoul_53

Waterfoul_54
(DnaQ exonuclease (DNA polymerase III subunit))

Waterfoul_55

Waterfoul_56

Waterfoul_57

Waterfoul_58

Waterfoul_59

Waterfoul_60

Waterfoul_61

Waterfoul_62

Waterfoul_63
(HNH endonuclease)

Waterfoul_64
(HNH endonuclease)

Waterfoul_65
(DNA primase/polymerase)

Waterfoul_66
(DNA helicase)

Waterfoul_67
(RusA resolvase (endonuclease))

Waterfoul_68

Waterfoul_69

Waterfoul_70

Waterfoul_71

Waterfoul_72

Waterfoul_73

Waterfoul_74

Waterfoul_75

Waterfoul_76

Waterfoul_77

Waterfoul_78
(RNA ligase)

Waterfoul_79

Waterfoul_80

Waterfoul_81

Waterfoul_82

Waterfoul_83

Waterfoul_84

Waterfoul_85

Waterfoul_86

Waterfoul_87

Waterfoul_88

Waterfoul_89

Waterfoul_90

Waterfoul_91

Waterfoul_92

Waterfoul_93

Waterfoul_94

Waterfoul_95
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank Accession KX585251
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload