Mycobacterium phage TreyKay
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Detailed Information for Phage TreyKay
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTrevor Khaba
Year Found2015
Location FoundPinetown, South africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.821222 S, 30.887583 E Map
Discovery NotesThe sample was collected from dry top soil in a flower garden. The soil had lots of roots and bits of dead plant. I collected the sample in the morning at around 7am. I used a plastic back to scoop it up and it was kept in my bag for a few hours before isolation.
Naming NotesI named the phage TreyKay as a play on my name and surname Trevor khaba. Trey being a nickname that I am sometimes called and kay as the first letter of my surname written out. Im not known for my creativity i would have just named it Trevorkhaba after myself but alas, rules. So to me it will always unofficially be the Trevorkhaba phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 16, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage968
Genome length (bp)60311
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.8%
Sequencing Notessame length but 2 nucleotide difference from Prithvi
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesThe plaques appear as small circular clearings, but after two days show the appearance of a halo. After three days they become larger and clouded.
MorphotypeSiphoviridae
Final annotation complete?Yes
Has been Phamerated?Yes
Gene List

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TreyKay_1

TreyKay_2

TreyKay_3

TreyKay_4

TreyKay_6

TreyKay_7

TreyKay_8
(terminase)

TreyKay_9
(portal protein)

TreyKay_10
(capsid maturation protein)

TreyKay_11

TreyKay_12
(scaffolding protein)

TreyKay_13
(major capsid protein)

TreyKay_14
(head-to-tail connector)

TreyKay_15
(head-to-tail connector)

TreyKay_16
(head-to-tail connector)

TreyKay_17
(head-to-tail connector)

TreyKay_18
(major tail protein)

TreyKay_19
(tail assembly chaperone)

TreyKay_20
(tail assembly chaperone)

TreyKay_21
(tape measure protein)

TreyKay_22
(minor tail protein)

TreyKay_23
(minor tail protein)

TreyKay_24
(minor tail protein)

TreyKay_25
(minor tail protein)

TreyKay_26
(minor tail protein)

TreyKay_27
(minor tail protein)

TreyKay_28
(minor tail protein)

TreyKay_29

TreyKay_30
(lysin A)

TreyKay_31
(lysin B)

TreyKay_32
(holin)

TreyKay_33

TreyKay_34

TreyKay_35
(exonuclease)

TreyKay_36

TreyKay_37

TreyKay_38

TreyKay_39
(queuine tRNA-ribosyltransferase)

TreyKay_40

TreyKay_41

TreyKay_42
(integrase)

TreyKay_43
(membrane protein)

TreyKay_44
(immunity repressor)

TreyKay_45

TreyKay_46
(helix-turn-helix DNA binding protein)

TreyKay_47

TreyKay_48

TreyKay_49

TreyKay_50
(helix-turn-helix DNA binding protein)

TreyKay_51
(WhiB family transcription factor)

TreyKay_52

TreyKay_53

TreyKay_54

TreyKay_55

TreyKay_56

TreyKay_57

TreyKay_58
(exonuclease)

TreyKay_59

TreyKay_60

TreyKay_61

TreyKay_62

TreyKay_63

TreyKay_64

TreyKay_65

TreyKay_66

TreyKay_67

TreyKay_68

TreyKay_69
(HNH endonuclease)

TreyKay_70
(DNA primase/polymerase)

TreyKay_71

TreyKay_72

TreyKay_73
(resolvase)

TreyKay_74

TreyKay_75

TreyKay_76
(helix-turn-helix domain protein)

TreyKay_77

TreyKay_78

TreyKay_79
(protease)

TreyKay_80

TreyKay_81

TreyKay_82

TreyKay_83

TreyKay_84

TreyKay_85

TreyKay_86

TreyKay_87
(RNA ligase)

TreyKay_88

TreyKay_89

TreyKay_90

TreyKay_91

TreyKay_92

TreyKay_93

TreyKay_94

TreyKay_95

TreyKay_96

TreyKay_97

TreyKay_98
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF472892
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload