Mycobacterium phage Collard
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Detailed Information for Phage Collard
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJasmine Jones
Year Found2011
Location FoundSaint Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.754444 N, 90.288611 W Map
Discovery NotesAir Temperature: 75 degrees Fahrenheit
Depth: 3.1 cm
Moisture Content: Dry
Found in an urban area
Location was NOT sloped
Obtained near/in a garden where collard greens and tomatoes are grown
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 8, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage198
Sanger Finishing Reactions8
Genome length (bp)61395
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesThe plaques are small, clear and circular. This description is relatively consistent on all plated phage. Average area of plaque is 12.6 mm^2.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Collard_1

Collard_2

Collard_3

Collard_5
(terminase small subunit)

Collard_6
(terminase large subunit)

Collard_7
(portal protein)

Collard_8
(capsid maturation protease)

Collard_9

Collard_10
(scaffolding protein)

Collard_11
(major capsid protein)

Collard_12
(head-to-tail connector complex protein)

Collard_13
(head-to-tail connector complex protein)

Collard_14

Collard_15
(head-to-tail connector complex protein)

Collard_16
(major tail protein)

Collard_17
(tail assembly chaperone)

Collard_18
(tail assembly chaperone)

Collard_19
(tape measure protein)

Collard_20
(minor tail protein)

Collard_21
(minor tail protein)

Collard_22
(minor tail protein)

Collard_23
(minor tail protein)

Collard_24

Collard_25
(minor tail protein)

Collard_26

Collard_27
(minor tail protein)

Collard_28

Collard_29
(lysin A)

Collard_30
(lysin B)

Collard_31
(holin)

Collard_32
(membrane protein)

Collard_33

Collard_34
(exonuclease)

Collard_35

Collard_36
(helix-turn-helix DNA binding domain protein)

Collard_37
(hydrolase)

Collard_38
(integrase)

Collard_39

Collard_40
(membrane protein)

Collard_41
(immunity repressor)

Collard_42
(Cro protein)

Collard_43

Collard_44
(membrane protein)

Collard_45

Collard_46

Collard_47
(membrane protein)

Collard_48

Collard_49

Collard_50
(membrane protein)

Collard_51

Collard_52

Collard_53
(exonuclease)

Collard_54
(membrane protein)

Collard_55

Collard_56

Collard_57

Collard_58
(NrdH-like glutaredoxin)

Collard_59
(HNH endonuclease)

Collard_60
(HNH endonuclease)

Collard_61
(DNA primase/polymerase)

Collard_62
(helicase)

Collard_63
(RusA-like resolvase)

Collard_64
(peptidase)

Collard_65

Collard_66

Collard_67
(membrane protein)

Collard_68

Collard_69

Collard_70

Collard_71

Collard_72

Collard_73
(membrane protein)

Collard_74
(RNA ligase)

Collard_75

Collard_76

Collard_77

Collard_78

Collard_79

Collard_80

Collard_81

Collard_82

Collard_83

Collard_84
(queuine tRNA-ribosyltransferase)

Collard_85

Collard_86

Collard_87

Collard_88

Collard_89

Collard_90

Collard_91

Collard_92

Collard_93

Collard_94

Collard_95
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH651171
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2011WUSLcollardKH
SEA Lysate Titer 1.46e8
Date of SEA Lysate Titering Oct 6, 2011
Pitt Freezer Box# 31
Pitt Freezer Box Grid# 26
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload