Mycobacterium phage Belladonna
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Detailed Information for Phage Belladonna
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySummer Wei
Year Found2016
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates36.986944 N, 86.454444 W Map
Discovery NotesThis sample was collected from some tree roots from outside of Florence Schneider Hall in WKU, Bowling Green, KY
Naming NotesBelladonna translates to "beautiful lady" and was used in Italy as an eye enhancer although it was also used as poison. The phage Belladonna, has a large brush, like a lady of the past.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3212
Genome length (bp)59708
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesCircular clear plaques, about 2mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Belladonna_1

Belladonna_2

Belladonna_3

Belladonna_4

Belladonna_6

Belladonna_7
(terminase, small subunit)

Belladonna_8
(terminase, large subunit)

Belladonna_9
(portal protein)

Belladonna_10
(capsid maturation protease)

Belladonna_11

Belladonna_12
(scaffolding protein)

Belladonna_13
(major capsid protein)

Belladonna_14
(head-to-tail connector complex protein)

Belladonna_15
(head-to-tail connector complex protein)

Belladonna_16
(head-to-tail connector complex protein)

Belladonna_17
(head-to-tail connector complex protein)

Belladonna_18
(major tail protein)

Belladonna_19
(tail assembly chaperone)

Belladonna_20
(tail assembly chaperone)

Belladonna_21
(tape measure protein)

Belladonna_22
(minor tail protein)

Belladonna_23
(minor tail protein)

Belladonna_24
(minor tail protein)

Belladonna_25
(minor tail protein)

Belladonna_26
(minor tail protein)

Belladonna_27
(minor tail protein)

Belladonna_28
(minor tail protein)

Belladonna_29

Belladonna_30
(lysin A)

Belladonna_31
(lysin B)

Belladonna_32
(holin)

Belladonna_33

Belladonna_34

Belladonna_35
(exonuclease)

Belladonna_36
(helix-turn-helix DNA binding domain)

Belladonna_37

Belladonna_38

Belladonna_39

Belladonna_40

Belladonna_41
(integrase, (Y-int))

Belladonna_42
(membrane protein)

Belladonna_43
(immunity repressor)

Belladonna_44
(Cro (control of repressor`s operator))

Belladonna_45
(excise)

Belladonna_46

Belladonna_47
(membrane protein)

Belladonna_48

Belladonna_49
(helix-turn-helix DNA binding domain)

Belladonna_50
(WhiB family transcription factor)

Belladonna_51

Belladonna_52

Belladonna_53

Belladonna_54

Belladonna_55

Belladonna_56

Belladonna_57
(DnaQ-like exonuclease (DNA polymerase III subunit))

Belladonna_58

Belladonna_59

Belladonna_60

Belladonna_61
(exonuclease)

Belladonna_62

Belladonna_63

Belladonna_64

Belladonna_65

Belladonna_66
(NrdH-like glutaredoxin)

Belladonna_67
(membrane protein)

Belladonna_68
(HNH endonuclease)

Belladonna_69
(DNA primase/polymerase)

Belladonna_70

Belladonna_71
(RusA-like resolvase (endonuclease))

Belladonna_72

Belladonna_73
(helix-turn-helix DNA binding domain)

Belladonna_74

Belladonna_75

Belladonna_76
(SprT-like protease)

Belladonna_77

Belladonna_78

Belladonna_79
(membrane protein)

Belladonna_80

Belladonna_81

Belladonna_82

Belladonna_83

Belladonna_84

Belladonna_85
(rtcB-like RNA ligase)

Belladonna_86

Belladonna_87

Belladonna_88

Belladonna_89

Belladonna_90

Belladonna_91
(antitoxin in toxin/antitoxin system, HicB-like)

Belladonna_92
(toxin in toxin/antitoxin system, HicA-like)

Belladonna_93

Belladonna_94

Belladonna_95

Belladonna_96

Belladonna_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH697578
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.2*10^10
Pitt Freezer Box# 37
Pitt Freezer Box Grid# B4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload