Mycobacterium phage BoostSeason
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Detailed Information for Phage BoostSeason
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJohn Peroza
Year Found2017
Location FoundVero Beach, FL United States
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates27.622778 N, 80.401611 E Map
Discovery NotesSoil collected next to a canal. Sample was a mix of mostly darkish dirt, with some sand. Sample was collected from under a flowerbed, surrounded by insects of many kinds.
Naming NotesOne of my hobbies, which is racing cars.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 20, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1367
Genome length (bp)58078
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGCGGCAT
GC Content68.2%
Sequencing Notessample contains mixed population, 16% Cluster L3 (not complete sequence)
Cluster K2 contains 12bp differences across genome, possibly 2 very similar phages
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK2
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesPlaques were circular, varying in size. Plaques were spread unequally throughout the plate, but with enough space between them for easy identification and quantification.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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BoostSeason_1

BoostSeason_2

BoostSeason_3

BoostSeason_4
(terminase)

BoostSeason_5
(portal protein)

BoostSeason_6
(capsid maturation protease)

BoostSeason_7

BoostSeason_8
(scaffolding protein)

BoostSeason_9
(major capsid protein)

BoostSeason_10
(head-to-tail adaptor)

BoostSeason_11
(head-to-tail stopper)

BoostSeason_12

BoostSeason_13
(tail terminator)

BoostSeason_14
(major tail protein)

BoostSeason_15
(tail assembly chaperone)

BoostSeason_16
(tail assembly chaperone)

BoostSeason_17
(tape measure protein)

BoostSeason_18
(minor tail protein)

BoostSeason_19
(minor tail protein)

BoostSeason_20
(minor tail protein)

BoostSeason_21
(minor tail protein)

BoostSeason_22
(minor tail protein)

BoostSeason_23
(minor tail protein)

BoostSeason_24
(minor tail protein)

BoostSeason_25
(minor tail protein)

BoostSeason_26

BoostSeason_27

BoostSeason_28

BoostSeason_29
(lysin A)

BoostSeason_30
(lysin B)

BoostSeason_31
(holin)

BoostSeason_32

BoostSeason_33

BoostSeason_34

BoostSeason_35
(exonuclease)

BoostSeason_36
(helix-turn-helix DNA binding domain)

BoostSeason_37

BoostSeason_38

BoostSeason_39

BoostSeason_40

BoostSeason_41

BoostSeason_42
(ssDNA binding protein)

BoostSeason_43
(queuine tRNA-ribosyltransferase)

BoostSeason_44
(integrase, (Y-int))

BoostSeason_45

BoostSeason_46
(immunity repressor)

BoostSeason_47
(Cro (control of repressor`s operator))

BoostSeason_48
(excise)

BoostSeason_49

BoostSeason_50

BoostSeason_51

BoostSeason_52

BoostSeason_53

BoostSeason_54
(WhiB family transcription factor)

BoostSeason_55
(hydrolase)

BoostSeason_56

BoostSeason_57

BoostSeason_58

BoostSeason_59

BoostSeason_60
(DnaQ-like exonuclease (DNA polymerase III subunit))

BoostSeason_61
(hydrolase)

BoostSeason_62
(exonuclease)

BoostSeason_63

BoostSeason_64

BoostSeason_65

BoostSeason_66

BoostSeason_67

BoostSeason_68

BoostSeason_69
(NrdH-like glutaredoxin)

BoostSeason_70

BoostSeason_71
(HNH endonuclease)

BoostSeason_72
(DNA primase)

BoostSeason_73
(RusA-like resolvase (endonuclease))

BoostSeason_74

BoostSeason_75

BoostSeason_76
(helix-turn-helix DNA binding domain)

BoostSeason_77

BoostSeason_78

BoostSeason_79

BoostSeason_80

BoostSeason_81
(SprT-like protease)

BoostSeason_82
(helix-turn-helix DNA binding domain)

BoostSeason_83

BoostSeason_84

BoostSeason_85

BoostSeason_86

BoostSeason_87

BoostSeason_88

BoostSeason_89

BoostSeason_90

BoostSeason_91

BoostSeason_92

BoostSeason_93

BoostSeason_94
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH834601
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 55
Pitt Freezer Box Grid# A8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload