Mycobacterium phage ShedlockHolmes
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Detailed Information for Phage ShedlockHolmes
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKathleen Shedlock
Year Found2014
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.439444 N, 79.946389 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 7, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage343
Genome length (bp)61081
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGATGGCAT
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK3
Other Cluster Members

Click to View

Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesThe phage has two different plaque morphologies; one larger, haloed turbid plaque and another that is a tiny pinprick.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

ShedlockHolmes_1

ShedlockHolmes_2

ShedlockHolmes_3

ShedlockHolmes_4

ShedlockHolmes_5

ShedlockHolmes_6
(small subunit)

ShedlockHolmes_7
(terminase, large subunit)

ShedlockHolmes_8
(portal)

ShedlockHolmes_9
(capsid maturation protease)

ShedlockHolmes_10

ShedlockHolmes_11
(scaffolding)

ShedlockHolmes_12
(major capsid protein)

ShedlockHolmes_13
(head to tail connector)

ShedlockHolmes_14
(head to tail connector)

ShedlockHolmes_15
(head to tail connector)

ShedlockHolmes_16
(head to tail connector)

ShedlockHolmes_17
(major tail subunit)

ShedlockHolmes_18
(tail assembly chaperone)

ShedlockHolmes_19
(tail assembly chaperone)

ShedlockHolmes_20
(tapemeasure)

ShedlockHolmes_21
(minor tail protein)

ShedlockHolmes_22
(minor tail protein)

ShedlockHolmes_23
(minor tail protein)

ShedlockHolmes_24
(minor tail protein)

ShedlockHolmes_25
(minor tail protein)

ShedlockHolmes_26
(minor tail protein)

ShedlockHolmes_27
(minor tail protein)

ShedlockHolmes_28
(minor tail protein)

ShedlockHolmes_29

ShedlockHolmes_30

ShedlockHolmes_31
(LysA)

ShedlockHolmes_32
(LysB)

ShedlockHolmes_33
(holin)

ShedlockHolmes_34

ShedlockHolmes_35

ShedlockHolmes_36

ShedlockHolmes_37

ShedlockHolmes_38

ShedlockHolmes_39

ShedlockHolmes_40

ShedlockHolmes_41

ShedlockHolmes_42

ShedlockHolmes_43
(integrase (Y-int))

ShedlockHolmes_44

ShedlockHolmes_45

ShedlockHolmes_46
(immunity repressor)

ShedlockHolmes_47
(CRO, HTH DNA binding domain)

ShedlockHolmes_48
(Xis (excise))

ShedlockHolmes_49

ShedlockHolmes_50

ShedlockHolmes_51

ShedlockHolmes_52

ShedlockHolmes_53

ShedlockHolmes_54
(WhiB)

ShedlockHolmes_55

ShedlockHolmes_56

ShedlockHolmes_57

ShedlockHolmes_58
(DnaQ)

ShedlockHolmes_59

ShedlockHolmes_60

ShedlockHolmes_61
(exonuclease)

ShedlockHolmes_62

ShedlockHolmes_63

ShedlockHolmes_64

ShedlockHolmes_65

ShedlockHolmes_66

ShedlockHolmes_67

ShedlockHolmes_68
(glutaredoxsin)

ShedlockHolmes_69

ShedlockHolmes_70

ShedlockHolmes_71
(DNA primase/helicase)

ShedlockHolmes_72

ShedlockHolmes_73
(RusA)

ShedlockHolmes_74

ShedlockHolmes_75

ShedlockHolmes_76
(peptidase)

ShedlockHolmes_77

ShedlockHolmes_78

ShedlockHolmes_79

ShedlockHolmes_80

ShedlockHolmes_81

ShedlockHolmes_82

ShedlockHolmes_83

ShedlockHolmes_84
(SprT)

ShedlockHolmes_85

ShedlockHolmes_86

ShedlockHolmes_87

ShedlockHolmes_88

ShedlockHolmes_89

ShedlockHolmes_90

ShedlockHolmes_91

ShedlockHolmes_92

ShedlockHolmes_93

ShedlockHolmes_94

ShedlockHolmes_95

ShedlockHolmes_96

ShedlockHolmes_97

ShedlockHolmes_98

ShedlockHolmes_99

ShedlockHolmes_100
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKR080206
Refseq NumberNone yet
Published in PaperPope WH et al 2015, Genome Announcements
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload