Mycobacterium phage Deby
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Detailed Information for Phage Deby
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByYiwei Sun
Year Found2017
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.039394 N, 118.4423 W Map
Discovery NotesPhage Deby was isolated by Yiwei Sun on 04/8/2017 from moist soil collected in an urban area, directly beneath metal pipes, next to building and pedestrian walk. Phage Deby was isolated by enrichment plating at 30C.

Characterization was performed by Team (KR)^2 (Keeyon Dabirian, Rachel Desai, Kyerra Jones, Ryan Toan Ngo) and Phantastic Phour (Diane Park, Erin Sakaji, Yiwei Sun, Boon Xin Tan).

Name of Individual who collected sample: Yiwei Sun
Midi-Lysate (Archive) Titer: 7x1010 PFU/ml
Date of Midi-Lysate Titer: 05/16/2017

AL Quarter and Lab section (ie. S15-1A): S17-1B
Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Sam Wu
Naming NotesThis phage was named "Deby" because it is an acronym of the Phantastic Phour team members' names.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 29, 2017
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)60463
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque Noteshalo (clear center with turbid edges)
Average size (and size range) diameter in mm: 3.82± 0.69mm ; 3mm-4mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Deby_1

Deby_2

Deby_3

Deby_4

Deby_6

Deby_7
(terminase, small subunit)

Deby_8
(terminase, large subunit)

Deby_9
(portal protein)

Deby_10
(capsid maturation protease)

Deby_11

Deby_12
(scaffolding protein)

Deby_13
(major capsid protein)

Deby_14
(head-to-tail connector complex protein)

Deby_15
(head-to-tail connector complex protein)

Deby_16

Deby_17
(head-to-tail connector complex protein)

Deby_18
(major tail protein)

Deby_19
(tail assembly chaperone)

Deby_20
(tail assembly chaperone)

Deby_21
(tape measure protein)

Deby_22
(minor tail protein)

Deby_23
(minor tail protein)

Deby_24
(minor tail protein)

Deby_25
(minor tail protein)

Deby_26
(minor tail protein)

Deby_27
(minor tail protein)

Deby_28
(minor tail protein)

Deby_29

Deby_30
(lysin A)

Deby_31
(lysin B)

Deby_32
(holin)

Deby_33

Deby_34

Deby_35
(exonuclease)

Deby_36
(helix-turn-helix DNA binding domain)

Deby_37

Deby_38

Deby_39

Deby_40

Deby_41
(integrase, (Y-int))

Deby_42
(membrane protein)

Deby_43
(immunity repressor)

Deby_44
(CRO (control of repressor s operator))

Deby_45
(helix-turn-helix DNA binding domain)

Deby_46

Deby_47
(membrane protein)

Deby_48

Deby_49
(helix-turn-helix DNA binding domain)

Deby_50
(WhiB family transcription factor)

Deby_51

Deby_52

Deby_53

Deby_54

Deby_55

Deby_56

Deby_57
(DnaQ exonuclease (DNA polymerase III subunit))

Deby_58

Deby_59

Deby_60

Deby_61
(exonuclease)

Deby_62

Deby_63

Deby_64

Deby_65

Deby_66

Deby_67
(NrdH glutaredoxin)

Deby_68
(HNH endonuclease)

Deby_69
(DNA primase/polymerase)

Deby_70
(RusA resolvase (endonuclease))

Deby_71

Deby_72

Deby_73

Deby_74

Deby_75

Deby_76
(SprT protease)

Deby_77

Deby_78

Deby_79
(membrane protein)

Deby_80

Deby_81

Deby_82

Deby_83

Deby_84

Deby_85
(rtcB RNA ligase)

Deby_86

Deby_87

Deby_88

Deby_89
(helix-turn-helix DNA binding domain)

Deby_90

Deby_91
(antitoxin in toxin/antitoxin system, HicB-like)

Deby_92
(toxin in toxin/antitoxin system, HicA-like)

Deby_93

Deby_94

Deby_95

Deby_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962364
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload