Mycobacterium phage Validus
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Detailed Information for Phage Validus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByValerie Briell
Year Found2012
Location FoundSeguin, TX USA
Finding InstitutionBaylor University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.57381 N, 97.816793 W Map
Discovery NotesSample site was a shaded dry creek bed. Sample was taken from a depth of 6 inches.
Naming NotesThe name Valerie is derived from the latin word Validus, meaning: strong, mighty, powerful, exceeding. The founders name is Valerie and so the phage is name Validus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage550
Sanger Finishing Reactions10
Genome length (bp)62466
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTGGGCAT
GC Content68.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesThe plaques have a clear center and a thick, hazy perimeter. Total diameter of plaque is approximately 10mm.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Validus_1

Validus_2

Validus_3

Validus_4

Validus_6

Validus_7
(Terminase small subunit)

Validus_8
(Terminase, large subunit)

Validus_9
(Portal)

Validus_10
(capsid maturation protease)

Validus_11

Validus_12
(scaffolding)

Validus_13
(Major Capsid)

Validus_14

Validus_15

Validus_16

Validus_17

Validus_18
(Major tail subunit)

Validus_19
(Tail assembly chaperone)

Validus_20
(Tail assembly chaperone)

Validus_21
(Tapemeasure)

Validus_22

Validus_23
(Minor Tail Protein)

Validus_24
(Minor Tail Protein)

Validus_25
(Minor Tail Protein)

Validus_26

Validus_27

Validus_28

Validus_29

Validus_30
(Lysin A)

Validus_31
(Lysin B)

Validus_32
(Holin)

Validus_33

Validus_34

Validus_35

Validus_36

Validus_37

Validus_38

Validus_39

Validus_40

Validus_41

Validus_42

Validus_43

Validus_44

Validus_45
(Integrase Y-int)

Validus_46

Validus_47
(immunity repressor)

Validus_48
(HTH DNA binding)

Validus_49
(HTH DNA binding)

Validus_50

Validus_51

Validus_52

Validus_53

Validus_54

Validus_55
(WhiB-like)

Validus_56

Validus_57

Validus_58

Validus_59

Validus_60

Validus_61
(DnaQ-like Protein)

Validus_62

Validus_63

Validus_64

Validus_65

Validus_66

Validus_67

Validus_68

Validus_69

Validus_70

Validus_71
(NrdH-like)

Validus_72

Validus_73

Validus_74
(DNA Primase)

Validus_75

Validus_76
(RusA-like)

Validus_77

Validus_78

Validus_79

Validus_80

Validus_81
(Putative PE family protein)

Validus_82
(SprT-like)

Validus_83

Validus_84

Validus_85

Validus_86

Validus_87

Validus_88

Validus_89

Validus_90

Validus_91
(RtcB-like)

Validus_92

Validus_93

Validus_94

Validus_95

Validus_96

Validus_97

Validus_98

Validus_99
(YcfA-like)

Validus_100

Validus_101

Validus_102

Validus_103

Validus_104

Validus_105

Validus_106
(HNH Endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF713486
Refseq NumberNC_023498
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload