Mycobacterium phage Milly
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Detailed Information for Phage Milly
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDanielle Lutyk & Oluwaseun Oyewole
Year Found2013
Location FoundMilledgeville, GA USA
Finding InstitutionGeorgia College and State University
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.119425 N, 83.185042 W Map
Discovery NotesSample Condition: 72°F, sunny. Samples collected from damp, shaded soil, approximately 20 m from shore of Lake Laurel
Naming NotesThe students who discovered Milly, named it after the town in which it was isolated, Milledgeville.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 29, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage253
Sanger Finishing Reactions23
Genome length (bp)58211
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGCGGCAT
GC Content68.3%
Sequencing NotesThere is a SNP at position 20606. The current call is G at that position, but about 30% of the reads have an A at that position instead.
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK2
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

Frankicide (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

Lazy (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Shrek (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

Lefty (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Humbert (K3)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

Mitti (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Cain (K6)

DarthP (K6)

Hammy (K6)

Krueger (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Plaque NotesMean plaques size: 3 mm diameter.
Plaques were uniformly small and clear.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes93
Has been Phamerated?Yes
Gene List

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Milly_1

Milly_2

Milly_3

Milly_4
(terminase)

Milly_5
(portal protein)

Milly_6
(capsid maturation protease)

Milly_7

Milly_8
(scaffolding protein)

Milly_9
(major capsid protein)

Milly_10

Milly_11

Milly_12

Milly_13

Milly_14
(major tail protein)

Milly_15
(tail assembly chaperone)

Milly_16
(tail assembly chaperone)

Milly_17
(tapemeasure)

Milly_18
(minor tail protein)

Milly_19
(minor tail protein)

Milly_20
(minor tail protein)

Milly_21
(minor tail protein)

Milly_22
(minor tail protein)

Milly_23
(minor tail protein)

Milly_24
(minor tail protein)

Milly_25
(minor tail protein)

Milly_26

Milly_27

Milly_28
(lysin A)

Milly_29
(lysin B)

Milly_30
(holin)

Milly_31

Milly_32

Milly_33

Milly_34

Milly_35

Milly_36
(transcription factor)

Milly_37

Milly_38

Milly_39

Milly_40

Milly_41
(DNA binding domain protein)

Milly_42
(queuine tRNA-ribosyltransferase)

Milly_43
(integrase)

Milly_44

Milly_45

Milly_46
(immunity repressor)

Milly_47
(transcription regulator)

Milly_48
(Xis)

Milly_49

Milly_50

Milly_51

Milly_52

Milly_53

Milly_54
(WhiB-like protein)

Milly_55
(hydrolase domain protein)

Milly_56

Milly_57

Milly_58

Milly_59

Milly_60
(DnaQ)

Milly_61
(hydrolase)

Milly_62
(exonuclease)

Milly_63

Milly_64

Milly_65

Milly_66

Milly_67

Milly_68

Milly_69
(NrdH-like glutaredoxin)

Milly_70

Milly_71
(HNH endonuclease)

Milly_72
(DNA primase/helicase)

Milly_73

Milly_74
(RusA)

Milly_75

Milly_76

Milly_77
(DNA binding domain protein)

Milly_78

Milly_79

Milly_80

Milly_81

Milly_82
(SprT-like protein)

Milly_83

Milly_84

Milly_85

Milly_86

Milly_87

Milly_88

Milly_89

Milly_90

Milly_91

Milly_92

Milly_93

Milly_94
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKP027206
Refseq NumberNC_026598
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload