Mycobacterium phage Paola
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Paola
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPaola G. Nol Bernardino
Year Found2017
Location FoundBellflower, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates33.896806 N, 118.124 W Map
Discovery NotesPhage Paola was isolated from moist soil collected at a depth of 1cm on 4/11/17 by Paola G. Nol Bernardino. Air temp at time of collection was 73F. Phage Paola was isolated via enrichment culture at 35C.

Name of Individual who collected sample: Paola G. Nol Bernardino
Midi-Lysate (Archive) Titer: 3.8 x 10e7 PFU/ml
Date of Midi-Lysate Titer: 4/27/17

MIMG103AL-S17 Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Ximena Ibarra
Undergraduate Assistant: Amara Pang
Laboratory Support: Dr. Krisanavane Reddi
Naming NotesNamed after the student who isolated the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)61535
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

Click to View

Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque Notesit was very particular because it was incredibly turbid and very hard to see at first. In the MIDI lysate the plaques were very large and turbid, more halo like.
Average size (and size range) diameter in mm: 1.5 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Paola_1

Paola_2

Paola_3

Paola_5
(terminase small subunit)

Paola_6
(terminase large subunit)

Paola_7
(portal protein)

Paola_8
(capsid maturation protease)

Paola_9

Paola_10
(scaffolding protein)

Paola_11
(major capsid protein)

Paola_12
(head-to-tail connector complex protein)

Paola_13
(head-to-tail connector complex protein)

Paola_14

Paola_15
(head-to-tail connector complex protein)

Paola_16
(major tail protein)

Paola_17
(tail assembly protein)

Paola_18
(tail assembly chaperone)

Paola_19
(tapemeasure protein)

Paola_20
(minor tail protein)

Paola_21
(minor tail protein)

Paola_22
(minor tail protein)

Paola_23
(minor tail protein)

Paola_24
(minor tail protein)

Paola_25
(minor tail protein)

Paola_26

Paola_27
(minor tail)

Paola_28

Paola_29
(lysin A)

Paola_30
(lysin B)

Paola_31
(holin)

Paola_32
(membrane domain protein)

Paola_33

Paola_34
(exonuclease)

Paola_35

Paola_36

Paola_37
(helix-turn-helix DNA binding protein)

Paola_38
(integrase)

Paola_39

Paola_40
(membrane protein)

Paola_41
(immunity repressor)

Paola_42
(CRO protein)

Paola_43

Paola_44
(membrane protein)

Paola_45
(helix-turn-helix DNA binding protein)

Paola_46

Paola_47
(DNA polymerase III sliding clamp beta)

Paola_48
(membrane domain protein)

Paola_49

Paola_50

Paola_51
(membrane domain protein)

Paola_52
(exonuclease)

Paola_53

Paola_54

Paola_55
(exonuclease)

Paola_56

Paola_57

Paola_58

Paola_59

Paola_60
(glutaredoxin)

Paola_61
(HNH endonuclease)

Paola_62
(HNH endonuclease)

Paola_63
(DNA primase/polymerase)

Paola_64
(DNA helicase)

Paola_65
(resolvase)

Paola_66

Paola_67

Paola_68

Paola_69
(membrane protein)

Paola_70

Paola_71

Paola_72

Paola_73

Paola_74

Paola_75

Paola_76

Paola_77
(membrane protein)

Paola_78
(RNA ligase)

Paola_79

Paola_80

Paola_81

Paola_82

Paola_83

Paola_84

Paola_85
(queuine tRNA-ribosyltransferase)

Paola_86
(BrnT-like toxin)

Paola_87
(RelB-like antitoxin)

Paola_88

Paola_89

Paola_90

Paola_91

Paola_92

Paola_93
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962374
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D6
Available Files
GenBank File for PhameratorDownload