Mycobacterium phage SgtBeansprout
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Detailed Information for Phage SgtBeansprout
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySamantha Mosier
Year Found2016
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature35°C
GPS Coordinates34.068242 N, 118.442184 W Map
Discovery NotesSgtBeansprout was isolated from a moist and oily soil sample (traffic, human/animal hair observed nearby) on 3/30/16. Isolation and characterization was done by Teams Nicholas Phage (Christina Fournier, Christy Kim, Ivonne Romero, Mikael Sanchez) and Agarvated Buns and Burners (Nam Do, Samantha Mosier, Joyce Wang, Sam Wu). Annotation performed by team Plaque It Up (Rina Watanabe, O. Monifa Sawyerr, Carly Pierson.) UA: Emily Xu. TA: Emma Goodwin. Instructors: Dr. Francis Mercer and Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Annotation QC by Andrew Lund.
Naming NotesSgtBeansprout was so named because under the electron microscope, the phage looked like a bean sprout due to its long striated tail. Also, many capsid heads separated from their tails depicting a field resembling that of war. Therefore, the phage was named SgtBeanSprout.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)56439
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesClear and round plaques, 4.615±0.95mm in diameter
MorphotypeSiphoviridae
Number of Genes105
Number of tRNAs1
Has been Phamerated?Yes
Gene List

Click to View

SgtBeansprout_1

SgtBeansprout_2

SgtBeansprout_3
(terminase small subunit)

SgtBeansprout_4
(terminase large subunit)

SgtBeansprout_5
(portal protein)

SgtBeansprout_6
(capsid maturation protease)

SgtBeansprout_7

SgtBeansprout_8
(scaffolding protein)

SgtBeansprout_9
(major capsid protein)

SgtBeansprout_10
(head-to-tail connector complex protein)

SgtBeansprout_11
(head-to-tail connector complex protein)

SgtBeansprout_12
(head-to-tail connector complex protein)

SgtBeansprout_13
(head-to-tail connector complex protein)

SgtBeansprout_14
(major tail protein)

SgtBeansprout_15
(tail assembly chaperone)

SgtBeansprout_16
(tail assembly chaperone)

SgtBeansprout_17
(tape measure protein)

SgtBeansprout_18
(minor tail protein)

SgtBeansprout_19
(minor tail protein)

SgtBeansprout_20
(minor tail protein)

SgtBeansprout_21
(minor tail protein)

SgtBeansprout_22
(minor tail protein)

SgtBeansprout_23
(minor tail protein)

SgtBeansprout_24
(minor tail protein)

SgtBeansprout_25

SgtBeansprout_26
(lysin A)

SgtBeansprout_27
(lysin B)

SgtBeansprout_28
(holin)

SgtBeansprout_29

SgtBeansprout_30

SgtBeansprout_31
(exonuclease)

SgtBeansprout_32
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_33

SgtBeansprout_34

SgtBeansprout_35
(queuine tRNA-ribosyltransferase)

SgtBeansprout_36
(integrase)

SgtBeansprout_37
(membrane protein)

SgtBeansprout_38
(immunity repressor)

SgtBeansprout_39
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_40
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_41

SgtBeansprout_42

SgtBeansprout_43

SgtBeansprout_44

SgtBeansprout_45
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_46
(WhiB family transcription factor)

SgtBeansprout_47

SgtBeansprout_48

SgtBeansprout_49
(DNA polymerase III subunit)

SgtBeansprout_50
(exonuclease)

SgtBeansprout_51

SgtBeansprout_52

SgtBeansprout_53

SgtBeansprout_54

SgtBeansprout_55

SgtBeansprout_56
(glutaredoxin)

SgtBeansprout_57
(HNH endonuclease)

SgtBeansprout_58
(DNA primase/polymerase)

SgtBeansprout_59
(resolvase)

SgtBeansprout_60

SgtBeansprout_61
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_62

SgtBeansprout_63

SgtBeansprout_64

SgtBeansprout_65

SgtBeansprout_66
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_67
(phosphatase)

SgtBeansprout_68
(RNA ligase)

SgtBeansprout_69

SgtBeansprout_70

SgtBeansprout_71

SgtBeansprout_72

SgtBeansprout_73
(HicB-like antitoxin)

SgtBeansprout_74
(HicA-like toxin)

SgtBeansprout_75

SgtBeansprout_76
(helix-turn-helix DNA binding domain protein)

SgtBeansprout_77

SgtBeansprout_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH020245
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# E5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload