Mycobacterium phage Biglebops
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Detailed Information for Phage Biglebops
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBraden Bigelow
Year Found2016
Location FoundLondon, KY USA
Finding InstitutionSomerset Community College
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.129 N, 84.0833 W Map
Discovery Notes12 inches below ground in garden
He was born in a garden after a really big snow break
Found in some mushy wet soil

Naming NotesI named him this because his name is amazing
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage270
Genome length (bp)56454
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesClear Pretty circular
Very tiny had to have a lot to fill my plate
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Biglebops_1

Biglebops_2

Biglebops_3
(Terminase, small subunit)

Biglebops_4
(terminase, large subunit)

Biglebops_5
(portal protein)

Biglebops_6
(capsid maturation protease)

Biglebops_7

Biglebops_8
(scaffolding protein)

Biglebops_9
(major capsid protein)

Biglebops_10
(head-to-tail connector protein)

Biglebops_11
(head-to-tail connector protein)

Biglebops_12
(head-to-tail connector protein)

Biglebops_13
(head-to-tail connector protein)

Biglebops_14
(major tail protein)

Biglebops_15
(tail assembly chaperone)

Biglebops_16
(tail assembly chaperone)

Biglebops_17
(tape measure protein)

Biglebops_18
(Minor tail protein)

Biglebops_19
(Minor tail protein)

Biglebops_20
(Minor tail protein)

Biglebops_21
(minor tail protein)

Biglebops_22
(Minor tail protein)

Biglebops_23
(Minor tail protein)

Biglebops_24
(Minor tail protein)

Biglebops_25

Biglebops_26
(lysin A)

Biglebops_27
(lysin B)

Biglebops_28
(holin)

Biglebops_29
(membrane protein)

Biglebops_30

Biglebops_31
(exonuclease)

Biglebops_32
(helix-turn-helix DNA binding domain)

Biglebops_33

Biglebops_34

Biglebops_35
(queuine tRNA-ribosyltransferase)

Biglebops_36
(integrase, (Y-int))

Biglebops_37
(membrane protein)

Biglebops_38
(Immunity Repressor)

Biglebops_39
(CRO (control of repressor operator))

Biglebops_40
(excise)

Biglebops_41

Biglebops_42

Biglebops_43
(membrane protein)

Biglebops_44

Biglebops_45
(helix-turn-helix DNA binding domain)

Biglebops_46
(WhiB family transcription factor)

Biglebops_47

Biglebops_48

Biglebops_49
(DnaQ exonuclease (DNA polymerase III subunit))

Biglebops_50
(exonuclease)

Biglebops_51

Biglebops_52

Biglebops_53

Biglebops_54

Biglebops_55
(NrdH glutaredoxin)

Biglebops_56
(HNH endonuclease)

Biglebops_57
(DNA primase)

Biglebops_58
(RusA resolvase (endonuclease))

Biglebops_59

Biglebops_60

Biglebops_61

Biglebops_62

Biglebops_63

Biglebops_64

Biglebops_65
(membrane protein)

Biglebops_66
(phosphatase)

Biglebops_67
(rtcB RNA ligase)

Biglebops_68

Biglebops_69

Biglebops_70

Biglebops_71

Biglebops_72
(antitoxin in toxin/antitoxin system, HicB-like)

Biglebops_73
(toxin in toxin/antitoxin system, HicB-like)

Biglebops_74

Biglebops_75

Biglebops_76

Biglebops_77
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399770
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.4x10^11 pfu/mL
Date of SEA Lysate Titering Apr 28, 2016
Pitt Freezer Box# 28
Pitt Freezer Box Grid# B1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload