Mycobacterium phage Urkel
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Detailed Information for Phage Urkel
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPerry Yazzolino & Douglas Kempthorne
Year Found2014
Location FoundSpokane, WA United States
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates47.66689 N, 117.4031 W Map
Discovery NotesThe sample was collected from the flower bed in front of the entrance to Hughes Hall. The flowers received direct sunlight; however, the origin of the sample received no sunlight because it was covered by flower petals.
Growth NotesIsolated at room temperature
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage115
Genome length (bp)60526
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesThere was no growth in the K lysogen spot plate - leading to the conclusion our phage is a part of the K cluster.
Other Cluster Members

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Aella (None)

AllaKazam (None)

Aloysius (None)

Andy (None)

Asayake (None)

Aspen (None)

Auroraborealis (None)

BackInNotBlack (None)

BaenaC (None)

Bailcon (None)

BasselJr (None)

Boogaloo (None)

Boyz (None)

Calcy (None)

Caroline (None)

Caspian (None)

Chat (None)

Cirrostratus (None)

Clarence (None)

Cookland (None)

Cortana (None)

Cunlay (None)

Dcuz (None)

Denara (None)

Dor110 (None)

DoubleSloth (None)

Dougal (None)

DrThomas (None)

DubyaDubya (None)

ElegantBeing (None)

Erinyes (None)

Fisch (None)

FitzSpotz (None)

GoBuffs (None)

GUsh (None)

GUsthebus (None)

Isa16 (None)

Jallie (None)

Jamanda (None)

Jameskc (None)

Kennel (None)

KevinPhangos (None)

LaFlor (None)

LanaDelPhay (None)

Lancelot (None)

LilPhil (None)

LuckyWorm (None)

MacArthur (None)

Machine (None)

MaeVan (None)

ManBun (None)

Marilu (None)

Marvolo (None)

McSparkle (None)

Meow (None)

Mileyphyrus (None)

Misa (None)

Mochi (None)

Nestitis (None)

NimNO (None)

Noonan (None)

Pablo (None)

PeachyKay (None)

Pegasicorn (None)

Phaedra (None)

Phaguccine (None)

Phlynn (None)

Pita107 (None)

Plaqccine (None)

Pluto15 (None)

Poochlock (None)

PresidentPhayn (None)

PresThayne (None)

PuddlePaige (None)

Reina (None)

Rivierearbre (None)

Sabotage (None)

Smore (None)

Speckles360 (None)

SpeedyClaxton (None)

Spots16 (None)

Stoya (None)

SuJos8 (None)

SwaggyP (None)

Syra333 (None)

Thayne (None)

Tomothy (None)

TrippyB (None)

TRossi (None)

Uilani (None)

Winton (None)

Wivia (None)

Yachna (None)

Yianni (None)

Zag19 (None)

Zagup (None)

Zhage (None)

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Atiba (K1)

BarrelRoll (K1)

Bash (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

Biglebops (K1)

Blessed (K1)

BlueSky (K1)

Capricorn (K1)

Chris (K1)

Christoria (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

DrHayes (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Erdnod (K1)

Flash (K1)

Gansey (K1)

Geraldini (K1)

Guanica15 (K1)

Guimauve (K1)

HedwigODU (K1)

Homura (K1)

JAWS (K1)

Jeckyll (K1)

Jerome (K1)

Jersey (K1)

Jophie (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

Lefty (K1)

Lela18 (K1)

LeMond (K1)

LeslieK (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mendoza (K1)

MicroMini (K1)

MLouK (K1)

Mozingo (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Oscar (K1)

Peanam (K1)

PhindingNemo (K1)

Phineas3776 (K1)

Pompey (K1)

Prithvi (K1)

Ramen (K1)

Rapunzel97 (K1)

Rozay (K1)

SamuelLPlaqson (K1)

Sarang (K1)

Scarlett (K1)

Schmutz (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Smurfee (K1)

Sulley (K1)

Tachez (K1)

TinkerBelle (K1)

TreyKay (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

Vidiian (K1)

Wilota (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BontFonne (K2)

BoostSeason (K2)

Cygnus24 (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Hyperbowlee (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Persephone (K2)

TM4 (K2)

Uncooperative (K2)

Zigg (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

RonKinser (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Chancellor (K4)

Cheetobro (K4)

Fionnbharth (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

NemNuong (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Euphorie (K6)

Hammy (K6)

Krueger (K6)

Lazy (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotation StatusIn GenBank
Plaque NotesUrkel was all uniform, circular and ranged in size from 0.1cm to 0.2 cm.
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs1
Has been Phamerated?Yes
Gene List

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Urkel_1

Urkel_2

Urkel_3

Urkel_4

Urkel_6

Urkel_7
(terminase small subunit)

Urkel_8
(terminase large subunit)

Urkel_9
(portal protein)

Urkel_10
(capsid maturation protease)

Urkel_11

Urkel_12
(scaffolding protein)

Urkel_13
(major capsid protein)

Urkel_14
(head-to-tail connector protein)

Urkel_15
(head-to-tail connector protein)

Urkel_16
(head-to-tail connector protein)

Urkel_17
(head-to-tail connector protein)

Urkel_18
(major tail protein)

Urkel_19
(tail assembly chaperone)

Urkel_20
(tail assembly chaperone)

Urkel_21
(tape measure protein)

Urkel_22
(minor tail protein)

Urkel_23
(minor tail protein)

Urkel_24
(minor tail protein)

Urkel_25
(minor tail protein)

Urkel_26

Urkel_27
(minor tail protein)

Urkel_28
(minor tail protein)

Urkel_29

Urkel_30
(lysin A)

Urkel_31
(lysin B)

Urkel_32
(holin)

Urkel_33

Urkel_34

Urkel_35

Urkel_36

Urkel_37

Urkel_38

Urkel_39

Urkel_40
(queuine tRNA-ribosyltransferase)

Urkel_41

Urkel_42
(integrase)

Urkel_43

Urkel_44
(immunity repressor)

Urkel_45
(Cro protein)

Urkel_46
(excise)

Urkel_47

Urkel_48

Urkel_49

Urkel_50
(helix-turn-helix DNA binding domain protein)

Urkel_51
(WhiB family transcription factor)

Urkel_52

Urkel_53

Urkel_54

Urkel_55

Urkel_56

Urkel_57

Urkel_58
(DNA polymerase III subunit)

Urkel_59

Urkel_60

Urkel_61

Urkel_62
(exonuclease)

Urkel_63

Urkel_64

Urkel_65

Urkel_66

Urkel_67
(glutaredoxin)

Urkel_68
(HNH endonuclease)

Urkel_69
(DNA primase)

Urkel_70

Urkel_71
(resolvase)

Urkel_72

Urkel_73
(helix-turn-helix DNA binding domain protein)

Urkel_74

Urkel_75

Urkel_76
(protease)

Urkel_77

Urkel_78

Urkel_79

Urkel_80
(helix-turn-helix DNA binding domain protein)

Urkel_81

Urkel_82

Urkel_83

Urkel_84

Urkel_85
(RNA ligase)

Urkel_86

Urkel_87

Urkel_88

Urkel_89

Urkel_90

Urkel_91

Urkel_92

Urkel_93

Urkel_94

Urkel_95

Urkel_96

Urkel_97

Urkel_98
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX657796
Refseq NumberNone yet
Published in PaperKirk Anders, Marianne K. Poxlietner 2017, Genome Announcements: Genome Sequences of Cluster K Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.7x10^8 pfu/mL
Pitt Freezer Box# 17
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
EM PictureDownload