| Detailed Information for Phage Adora |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Amanda Cremeans |
| Year Found | 2017 |
| Location Found | Beaver Falls, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 17°C |
| GPS Coordinates | 40.786215 N, 80.330441 W Map |
| Discovery Notes | Collection time: 10:55 am EST
Conditions: partly cloudy, but sunny blue sky, the previous day was warm and rainy.
Soil quality: very soft and moist from the rain, not clay-like or sandy, some organic material present.
Protocol:
Using a small, inside out, ziplock bag as a glove, I collected a handful of lose soil from the ground near a dumpster behind an auto shop near my home. I collected soil from the surface level to about an inch down in the ground. I wanted the freshest sample possible, so I collected the sample as I was leaving to go to the lab. The soil was stored at room temperature for about an hour and half before being prepared for the shaker. |
| Naming Notes | My grandmother's middle name is Adora and I have always loved this name. She turned 93 years old the same day that Adora was first isolated. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1075 |
| Genome length (bp) | 51827 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Adora particles have a haloed morphology. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK061406 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
G7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
