Gordonia phage WinkNick
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Detailed Information for Phage WinkNick
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByFound By: Emily Mohn, Isolated By: Kaitlyn Rogers
Year Found2020
Location FoundKirkwood, MO United States
Finding InstitutionSt. Louis Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.567667 N, 90.422083 W Map
Discovery NotesMohn collected the soil sample behind the greenhouse on top of a hill. The GPS coordinates are: 38°34’03.6”N, 90°25’19.5”W. It was very cold outside and there was about 3 inches of snow on the ground.
Naming NotesMy dog and significant others names merged together. It sounds cute and it has meaning to me.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage348
Genome length (bp)47259
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGCCAAGGGGA
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ4
Cluster Life CycleTemperate
Lysogeny NotesDNA concentration= 119.4 ng/ul
Average Capsid Width= 69.5 nm
Average Tail Length= 336.8 nm
Average Tail Width= 13.5 nm
Other Cluster Members
Annotating InstitutionSt. Louis Community College
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesPlaques were small (<1-2 mm) and rounded. Titer performed to 10^-8. Chose from titer of 10^-7, only two plagues overlapped, the rest were spread out; total of 6 plaques counted. Obtained that there are 2x10^10 phage per mL.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10^-8
Pitt Freezer Box# 127
Pitt Freezer Box Grid# C8
Available Files
EM PictureDownload