Gordonia phage Polly
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Detailed Information for Phage Polly
Discovery Information
Isolation HostGordonia terrae 3612
Found ByLili Sweeney
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4611 N, 79.954 W Map
Discovery NotesDiscovered on 1/26/17 from direct isolation using a soil sample collected from a planter on a 48°F, rainy day. Clonal population for lysate production obtained on 2/14/17.
Naming NotesNickname of my Great Grandmother, Pauline, which is also my middle name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1782
Genome length (bp)49780
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesClear plaques about 1mm in length, occasionally noticeably larger or smaller plaques are present.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK820642
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.3 x 10^10
Date of SEA Lysate Titering Mar 2, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload