Gordonia phage Polly
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Detailed Information for Phage Polly
Discovery Information
Isolation HostGordonia terrae 3612
Found ByLili Sweeney
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4611 N, 79.954 W Map
Discovery NotesDiscovered on 1/26/17 from direct isolation using a soil sample collected from a planter on a 48°F, rainy day. Clonal population for lysate production obtained on 2/14/17.
Naming NotesNickname of my Great Grandmother, Pauline, which is also my middle name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1782
Genome length (bp)49780
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesClear plaques about 1mm in length, occasionally noticeably larger or smaller plaques are present.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Polly_1
(terminase small subunit)

Polly_2
(terminase large subunit)

Polly_3

Polly_4
(portal protein)

Polly_5
(capsid maturation protease)

Polly_6
(major capsid protein)

Polly_7

Polly_8
(head-to-tail adaptor)

Polly_9
(head-to-tail stopper)

Polly_10

Polly_11
(tail terminator)

Polly_12
(major tail protein)

Polly_13

Polly_14
(tail assembly chaperone)

Polly_15
(tail assembly chaperone)

Polly_16
(lipoprotein)

Polly_17
(tape measure protein)

Polly_18
(minor tail protein)

Polly_19
(minor tail protein)

Polly_20
(lysin A)

Polly_21
(holin)

Polly_22
(membrane protein)

Polly_23
(membrane protein)

Polly_24
(lysin B)

Polly_25
(minor tail protein)

Polly_26

Polly_27
(minor tail protein)

Polly_28

Polly_29
(metalloprotease)

Polly_30
(membrane protein)

Polly_31

Polly_32
(helix-turn-helix DNA binding domain protein)

Polly_33

Polly_34

Polly_35

Polly_36
(integrase)

Polly_37

Polly_38
(DNA binding domain protein)

Polly_39
(helix-turn-helix DNA binding domain protein)

Polly_40
(metalloprotease)

Polly_41
(immunity repressor)

Polly_42
(helix-turn-helix DNA binding protein)

Polly_43

Polly_44

Polly_45
(antirepressor)

Polly_46

Polly_47
(helix-turn-helix DNA binding domain protein)

Polly_48
(membrane protein)

Polly_49

Polly_50

Polly_51

Polly_52

Polly_53
(RecE-like exonuclease)

Polly_54
(RecT-like ssDNA binding protein)

Polly_55

Polly_56

Polly_57

Polly_58
(ASC-1 transcription coactivator)

Polly_59
(membrane protein)

Polly_60
(DNA methylase)

Polly_61

Polly_62

Polly_63

Polly_64

Polly_65

Polly_66

Polly_67

Polly_68
(RusA-like resolvase)

Polly_69

Polly_70

Polly_71

Polly_72
(helix-turn-helix DNA binding domain protein)

Polly_73

Polly_74

Polly_75

Polly_76
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK820642
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.3 x 10^10
Date of SEA Lysate Titering Mar 2, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload