Gordonia phage Herod
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Detailed Information for Phage Herod
Discovery Information
Isolation HostGordonia terrae 3612
Former namesJohntheBactist
Found ByLuke Livingston and Riley Barnes
Year Found2017
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.1265 N, 93.048953 W Map
Discovery NotesIt was found in a mound of sand and silt on the bank of the Ouachita River on the campus of Ouachita Baptist University.
Naming NotesThis phage is named in honor of the first phage that my lab partner and I discovered. The first phage did not produce measureable plaques, nor did it produced a large quantity of plaques. We named it John the Bactist because after we first performed a spot test to see if any of our soil samples contained bacteriophages, we were told that we didn't have a phage. The next day in class, much to our surprise, we found out that we did in fact have a phage because there was a spot on the petri dish. We considered ourselves blessed with a phage that we were never supposed to have, much like Elizabeth and Zechariah in the Bible. Since we were unable to continue to work with this bacteriophage, but the name is completely awesome, we decided to keep the name for our second (hopefully more successful) phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 26, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2037
Genome length (bp)53826
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionOuachita Baptist University
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque Notesslightly cloudy, produces different plaque sizes.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.07x10^10 pfu/mL
Pitt Freezer Box# 48
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
EM PictureDownload