Gordonia phage Herod
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Detailed Information for Phage Herod
Discovery Information
Isolation HostGordonia terrae 3612
Former namesJohntheBactist
Found ByLuke Livingston and Riley Barnes
Year Found2017
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.1265 N, 93.048953 W Map
Discovery NotesIt was found in a mound of sand and silt on the bank of the Ouachita River on the campus of Ouachita Baptist University.
Naming NotesThis phage is named in honor of the first phage that my lab partner and I discovered. The first phage did not produce measureable plaques, nor did it produced a large quantity of plaques. We named it John the Bactist because after we first performed a spot test to see if any of our soil samples contained bacteriophages, we were told that we didn't have a phage. The next day in class, much to our surprise, we found out that we did in fact have a phage because there was a spot on the petri dish. We considered ourselves blessed with a phage that we were never supposed to have, much like Elizabeth and Zechariah in the Bible. Since we were unable to continue to work with this bacteriophage, but the name is completely awesome, we decided to keep the name for our second (hopefully more successful) phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 26, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2037
Genome length (bp)53826
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionOuachita Baptist University
Annotation StatusIn GenBank
Plaque Notesslightly cloudy, produces different plaque sizes.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Herod_1
(terminase small subunit)

Herod_2
(terminase large subunit)

Herod_3

Herod_4
(portal protein)

Herod_5
(capsid maturation protease)

Herod_6
(major capsid protein)

Herod_7

Herod_8
(head-to-tail adaptor)

Herod_9
(head-to-tail stopper)

Herod_10

Herod_11
(tail terminator)

Herod_12
(major tail protein)

Herod_13

Herod_14
(tail assembly chaperone)

Herod_15
(tail assembly chaperone)

Herod_16

Herod_17
(tape measure protein)

Herod_18
(minor tail protein)

Herod_19

Herod_20
(lysin A, L-Ala-D-Glu peptidase domain)

Herod_21
(lysin A, glycosyl hydrolase domain)

Herod_22
(holin)

Herod_23

Herod_24

Herod_25
(lysin B)

Herod_26
(minor tail protein)

Herod_27

Herod_28
(minor tail protein)

Herod_29

Herod_30

Herod_31

Herod_32

Herod_33

Herod_34
(helix-turn-helix DNA binding protein)

Herod_35

Herod_36

Herod_37

Herod_38

Herod_39
(tyrosine integrase)

Herod_40
(helix-turn-helix DNA binding protein)

Herod_41
(helix-turn-helix DNA binding protein)

Herod_42

Herod_43

Herod_44
(immunity repressor)

Herod_45
(helix-turn-helix DNA binding protein)

Herod_46

Herod_47

Herod_48
(antirepressor)

Herod_49

Herod_50
(helix-turn-helix DNA binding protein)

Herod_51

Herod_52

Herod_53

Herod_54

Herod_55

Herod_56

Herod_57
(RecE-like exonuclease)

Herod_58
(RecT-like ssDNA binding protein)

Herod_59

Herod_60

Herod_61

Herod_62

Herod_63
(RNA binding protein)

Herod_64

Herod_65

Herod_66

Herod_67
(DNA methylase)

Herod_68

Herod_69

Herod_70

Herod_71

Herod_72

Herod_73

Herod_74

Herod_75
(RusA-like resolvase)

Herod_76

Herod_77

Herod_78

Herod_79
(helix-turn-helix DNA binding protein)

Herod_80

Herod_81

Herod_82

Herod_83

Herod_84
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT889398
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.07x10^10 pfu/mL
Pitt Freezer Box# 48
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload