Gordonia phage Zameen
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Detailed Information for Phage Zameen
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJaspreet Singh
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44189 N, 79.9557 W Map
Discovery NotesCollected outside Barco Building in light brown (softball diamond) colored dirt next to a big maple tree.
Naming NotesMy family originates from Punjab, and so the name I chose, Zameen, is Punjabi for "earth" or "land." I chose this name because the soil sample came, literally, from the Earth. I also chose this name because it has a special personal significance to me - I am the first women in my family to pursue medicine and science research professionally. I wanted to chose a name that sounded pretty - (Phonetically it is ZUH - MEAN) but also showed how proud I am of my heritage and the opportunities I have today.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage346
Genome length (bp)50337
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesLysogen testing revealed that Zameen is likely a temperate phage, as it's plaques have hazy, rather than clear, edges.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe plaques on Zameen are round and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Zameen_1
(terminase, small subunit)

Zameen_2
(terminase, large subunit)

Zameen_3

Zameen_4
(portal protein)

Zameen_5
(capsid maturation protease)

Zameen_6
(major capsid protein)

Zameen_7

Zameen_8
(head-to-tail adaptor)

Zameen_9
(head-to-tail stopper)

Zameen_10

Zameen_11
(tail terminator)

Zameen_12
(major tail protein)

Zameen_13

Zameen_14
(tail assembly chaperone)

Zameen_15
(tail assembly chaperone)

Zameen_16

Zameen_17
(tape measure protein)

Zameen_18
(minor tail protein)

Zameen_19
(minor tail protein)

Zameen_20
(lysin A, L-Ala-D-Glu peptidase domain)

Zameen_21

Zameen_22
(lysin A, peptidase domain)

Zameen_23
(membrane protein)

Zameen_24
(membrane protein)

Zameen_25

Zameen_26
(membrane protein)

Zameen_27
(lysin B)

Zameen_28
(minor tail protein)

Zameen_29

Zameen_30
(minor tail protein)

Zameen_31

Zameen_32
(metalloprotease)

Zameen_33
(membrane protein)

Zameen_34

Zameen_35
(helix-turn-helix turn DNA binding domain)

Zameen_36

Zameen_37

Zameen_38

Zameen_39
(integrase (Y-Int))

Zameen_40

Zameen_41
(DNA binding domain)

Zameen_42
(helix-turn-helix DNA binding domain)

Zameen_43
(metallopeptidase)

Zameen_44
(immunity repressor)

Zameen_45
(helix-turn-helix DNA binding domain)

Zameen_46

Zameen_47

Zameen_48
(antirepressor)

Zameen_49

Zameen_50

Zameen_51
(membrane protein)

Zameen_52

Zameen_53

Zameen_54

Zameen_55

Zameen_56
(RecE-like exonuclease)

Zameen_57
(RecT-like ssDNA binding protein)

Zameen_58

Zameen_59

Zameen_60

Zameen_61

Zameen_62
(membrane protein)

Zameen_63
(DNA methylase)

Zameen_64

Zameen_65

Zameen_66

Zameen_67

Zameen_68

Zameen_69

Zameen_70

Zameen_71
(RusA-like resolvase (endonuclease))

Zameen_72

Zameen_73

Zameen_74

Zameen_75
(helix-turn-helix DNA binding domain)

Zameen_76

Zameen_77

Zameen_78

Zameen_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK878898
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 91
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload