Gordonia phage Zameen
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Detailed Information for Phage Zameen
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJaspreet Singh
Year Found2018
Location FoundPittsburgh, PA US
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44189 N, 79.9557 W Map
Discovery NotesCollected outside Barco Building in light brown (softball diamond) colored dirt next to a big maple tree.
Naming NotesMy family originates from Punjab, and so the name I chose, Zameen, is Punjabi for "earth" or "land." I chose this name because the soil sample came, literally, from the Earth. I also chose this name because it has a special personal significance to me - I am the first women in my family to pursue medicine and science research professionally. I wanted to chose a name that sounded pretty - (Phonetically it is ZUH - MEAN) but also showed how proud I am of my heritage and the opportunities I have today.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage346
Genome length (bp)50337
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesLysogen testing revealed that Zameen is likely a temperate phage, as it's plaques have hazy, rather than clear, edges.
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2019)
Plaque NotesThe plaques on Zameen are round and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload