Gordonia phage Tayonia
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Detailed Information for Phage Tayonia
Discovery Information
Isolation HostGordonia terrae 3612
Former namesTayoniaSII
Found ByTaylor Campbell
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.4425 N, 79.9225 W Map
Discovery NotesThe soil was collected in a flower bed on the Litchfield Towers Patio on campus at the University of Pittsburgh at 2:36 pm on 9/10/18. It was cloudy and raining at the time of collection. The soil was dark and moist. The collection site was mainly fresh soil with flowers and plants growing in the flower bed.
Naming NotesThe name is my own name mixed with the host and an initial of a last name with "II" meaning junior or the second.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage547
Genome length (bp)50320
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesAfter performing a spot test within the lysogeny protocol, mesas began forming on the first and second spots. Further testing must be completed to confirm lysogens.
Other Cluster Members
Annotation StatusQC Complete
Plaque NotesThe morphology for this phage seems to show up as two different clearings one small with a slightly smaller one also present. This pattern remained consistent through three rounds of purification.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10x10^11
Pitt Freezer Box# 91
Pitt Freezer Box Grid# F6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload