Gordonia phage Tayonia
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Detailed Information for Phage Tayonia
Discovery Information
Isolation HostGordonia terrae 3612
Former namesTayoniaSII
Found ByTaylor Campbell
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.4425 N, 79.9225 W Map
Discovery NotesThe soil was collected in a flower bed on the Litchfield Towers Patio on campus at the University of Pittsburgh at 2:36 pm on 9/10/18. It was cloudy and raining at the time of collection. The soil was dark and moist. The collection site was mainly fresh soil with flowers and plants growing in the flower bed.
Naming NotesThe name is my own name mixed with the host and an initial of a last name with "II" meaning junior or the second.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage547
Genome length (bp)50320
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesAfter performing a spot test within the lysogeny protocol, mesas began forming on the first and second spots. Further testing must be completed to confirm lysogens.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe morphology for this phage seems to show up as two different clearings one small with a slightly smaller one also present. This pattern remained consistent through three rounds of purification.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Tayonia_1
(terminase small subunit)

Tayonia_2
(terminase large subunit)

Tayonia_3
(membrane protein)

Tayonia_4
(portal protein)

Tayonia_5
(capsid maturation protease)

Tayonia_6
(major capsid protein)

Tayonia_7

Tayonia_8
(head-to-tail adaptor)

Tayonia_9
(head-to-tail stopper)

Tayonia_10

Tayonia_11
(tail terminator)

Tayonia_12
(major tail protein)

Tayonia_13

Tayonia_14
(tail assembly chaperone)

Tayonia_15
(tail assembly chaperone)

Tayonia_16

Tayonia_17
(tape measure protein)

Tayonia_18
(minor tail protein)

Tayonia_19
(minor tail protein)

Tayonia_20
(lysin A, L-Ala-D-Glu peptidase domain)

Tayonia_21

Tayonia_22
(lysin A, glycosyl hydrolase domain)

Tayonia_23
(membrane protein)

Tayonia_24
(membrane protein)

Tayonia_25

Tayonia_26
(membrane protein)

Tayonia_27
(lysin B)

Tayonia_28
(minor tail protein)

Tayonia_29

Tayonia_30
(minor tail protein)

Tayonia_31

Tayonia_32
(metalloprotease)

Tayonia_33
(membrane protein)

Tayonia_34

Tayonia_35
(helix-turn-helix DNA binding domain protein)

Tayonia_36
(membrane protein)

Tayonia_37
(membrane protein)

Tayonia_38

Tayonia_39
(integrase)

Tayonia_40

Tayonia_41
(DNA binding domain protein)

Tayonia_42
(helix-turn-helix DNA binding domain protein)

Tayonia_43
(metalloprotease)

Tayonia_44
(immunity repressor)

Tayonia_45
(helix-turn-helix DNA binding domain protein)

Tayonia_46

Tayonia_47

Tayonia_48
(antirepressor)

Tayonia_49

Tayonia_50

Tayonia_51
(membrane protein)

Tayonia_52

Tayonia_53

Tayonia_54

Tayonia_55

Tayonia_56
(RecE-like exonuclease)

Tayonia_57
(RecT-like ssDNA binding protein)

Tayonia_58

Tayonia_59

Tayonia_60

Tayonia_61
(ASC-1 transcription coactivator)

Tayonia_62
(membrane protein)

Tayonia_63
(DNA methylase)

Tayonia_64

Tayonia_65

Tayonia_66

Tayonia_67

Tayonia_68

Tayonia_69

Tayonia_70

Tayonia_71
(RusA-like resolvase)

Tayonia_72

Tayonia_73
(membrane protein)

Tayonia_74

Tayonia_75
(helix-turn-helix DNA binding domain protein)

Tayonia_76

Tayonia_77

Tayonia_78

Tayonia_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK875796
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10x10^11
Pitt Freezer Box# 91
Pitt Freezer Box Grid# F6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload