Gordonia phage Sekhmet
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Detailed Information for Phage Sekhmet
Discovery Information
Isolation HostGordonia terrae 3612
Found ByBrenden Johnson
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.442391 N, 79.952848 W Map
Discovery NotesWhen the sample yielding this phage was collected (9:52 AM on 9-12-18), the temperature was approximately 65 degrees Fahrenheit and the weather was cloudy. The soil was dark, indicating nutrient-richness, and the area surrounding the collection location was a commonly walked pathway with plenty of detritus but little vegetation.
Naming NotesSekhmet, in ancient Egyptian religion, was a warrior goddess whose name was often associated with rivalry and change. The rationale behind naming this particular phage Sekhmet is derived from the theory of a constant evolutionary battle between phages and their host bacteria. Just as bacteria have attained uncountable adaptations over their billions of years of coexistence with bacteriophages to prevent and deal with infection, phages have also adapted to more efficiently infect and propagate using their hosts. In this way, the population of bacteriophages is a symbol in biology much like Sekhmet was to the Egyptian people; wagers of war who trigger massive change in the world.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage501
Genome length (bp)47735
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGCCAAGGGGA
GC Content66.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are irregular but clear ranging from 0.5 and 3 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Sekhmet_1
(HNH endonuclease)

Sekhmet_2
(terminase small subunit)

Sekhmet_3
(terminase large subunit)

Sekhmet_4
(portal protein)

Sekhmet_5
(capsid maturation protease)

Sekhmet_6
(scaffolding protein)

Sekhmet_7
(major capsid protein)

Sekhmet_8

Sekhmet_9
(head-to-tail adaptor)

Sekhmet_10
(head-to-tail stopper)

Sekhmet_11

Sekhmet_12
(tail terminator)

Sekhmet_13
(major tail protein)

Sekhmet_14

Sekhmet_15
(tail assembly chaperone)

Sekhmet_16
(tail assembly chaperone)

Sekhmet_17

Sekhmet_18
(tape measure protein)

Sekhmet_19
(minor tail protein)

Sekhmet_20
(minor tail protein)

Sekhmet_21
(lysin A)

Sekhmet_22

Sekhmet_23

Sekhmet_24

Sekhmet_25
(lysin B)

Sekhmet_26
(minor tail protein)

Sekhmet_27

Sekhmet_28

Sekhmet_29

Sekhmet_30
(hydrolase)

Sekhmet_31
(acetyltransferase)

Sekhmet_32

Sekhmet_33

Sekhmet_34

Sekhmet_35

Sekhmet_36

Sekhmet_37
(tyrosine integrase)

Sekhmet_38

Sekhmet_39

Sekhmet_40
(tyrosine integrase)

Sekhmet_41
(helix-turn-helix DNA binding domain protein)

Sekhmet_42
(metalloprotease)

Sekhmet_43

Sekhmet_44
(immunity repressor)

Sekhmet_45
(helix-turn-helix DNA binding domain protein)

Sekhmet_46

Sekhmet_47
(antirepressor)

Sekhmet_48

Sekhmet_49

Sekhmet_50
(excise)

Sekhmet_51

Sekhmet_52

Sekhmet_53

Sekhmet_54

Sekhmet_55
(RecE-like exonuclease)

Sekhmet_56
(RecT-like ssDNA binding protein)

Sekhmet_57

Sekhmet_58

Sekhmet_59

Sekhmet_60

Sekhmet_61

Sekhmet_62
(helix-turn-helix DNA binding domain protein)

Sekhmet_63

Sekhmet_64

Sekhmet_65

Sekhmet_66

Sekhmet_67

Sekhmet_68

Sekhmet_69
(RusA-like resolvase)

Sekhmet_70

Sekhmet_71

Sekhmet_72

Sekhmet_73

Sekhmet_74

Sekhmet_75
(MerR-like helix-turn-helix DNA binding domain protein)

Sekhmet_76

Sekhmet_77

Sekhmet_78

Sekhmet_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK937609
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 10^10 pfu/mL
Pitt Freezer Box# 91
Pitt Freezer Box Grid# B4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload