Gordonia phage Dolores
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Detailed Information for Phage Dolores
Discovery Information
Isolation HostGordonia terrae 3612
Found ByRebecca Cohen
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.439423 N, 79.947033 W Map
Discovery NotesOn 1/10 the weather forecast said a high of 50 and a low of 25. It was lightly raining at 12:20pm when I walked to Phipps Conservatory, a public garden in Pittsburgh PA. I collected a soil sample from the walkway leading into the Conservatory. The GPS coordinates of the location were 40.439423 degrees North and 79.947033 degrees West. The soil was covered in mulch and very hard. I believe it was still slightly frozen from the day before which had a high of 29 and a low of 7. The soil sample was kept in my backpack until 4:30pm lab.
Naming NotesMy roommate was going to be named Dolores, which was an interesting topic of discussion around the time I discovered a phage. I named this phage Dolores in hopes that she would be an interesting topic of discussion.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 16, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage791
Genome length (bp)47336
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGCCAAGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMitchell Community College
Annotation StatusAwaiting_QC
Plaque NotesPlaques appear as a tiny, clear spots in the middle ~2mm with turbid halos surrounding them. Differences in halo sizes cause plaques to vary from ~3mm to 5 mm.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 55
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload