Gordonia phage Bialota
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Detailed Information for Phage Bialota
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAlexis Bialota
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.443172 N, 79.955837 W Map
Naming NotesMy last name
Mutants
Mutants of this Phage

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Bialota1A

MM100

MM101

MM102

MM103

MM104

Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 29, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3046
Genome length (bp)52089
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSome small plaques and large plaques were found, and after multiple serial dilutions, it was hypothesized that they were the same phage.
Has been Phamerated?Yes
Gene List

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Bialota_1
(terminase small subunit)

Bialota_2
(terminase large subunit)

Bialota_3

Bialota_4
(portal protein)

Bialota_5
(capsid maturation protease)

Bialota_6
(major capsid protein)

Bialota_7

Bialota_8
(head-to-tail adaptor)

Bialota_9
(head-to-tail stopper)

Bialota_10

Bialota_11
(tail terminator)

Bialota_12
(major tail protein)

Bialota_13

Bialota_14
(tail assembly chaperone)

Bialota_15
(tail assembly chaperone)

Bialota_16

Bialota_17
(tape measure protein)

Bialota_18
(minor tail protein)

Bialota_19
(minor tail protein)

Bialota_20
(lysin A, L-ala D-glu peptidase domain)

Bialota_21
(lysin A, glycosyl hydrolase domain)

Bialota_22

Bialota_23

Bialota_24

Bialota_25
(lysin B)

Bialota_26
(minor tail protein)

Bialota_27

Bialota_28
(minor tail protein)

Bialota_29

Bialota_30

Bialota_31

Bialota_32

Bialota_33

Bialota_34

Bialota_35

Bialota_36

Bialota_37
(integrase)

Bialota_38

Bialota_39

Bialota_40

Bialota_41
(DNA binding protein)

Bialota_42
(immunity repressor)

Bialota_43
(helix-turn-helix DNA binding domain protein)

Bialota_44

Bialota_45

Bialota_46
(antirepressor)

Bialota_47

Bialota_48

Bialota_49

Bialota_50

Bialota_51

Bialota_52

Bialota_53

Bialota_54
(RecE-like exonuclease)

Bialota_55
(RecT-like ssDNA binding protein)

Bialota_56

Bialota_57

Bialota_58

Bialota_59
(ASC-1 transcription coactivator)

Bialota_60

Bialota_61
(DNA methylase)

Bialota_62

Bialota_63

Bialota_64

Bialota_65

Bialota_66
(RepA-like helicase)

Bialota_67

Bialota_68

Bialota_69
(RusA-like resolvase)

Bialota_70

Bialota_71

Bialota_72

Bialota_73

Bialota_74
(excise)

Bialota_75

Bialota_76

Bialota_77

Bialota_78

Bialota_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016492
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.1x10^9
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload