Gordonia phage Bosnia
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Detailed Information for Phage Bosnia
Discovery Information
Isolation HostGordonia terrae 3612
Found ByLejla Suljevic
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature27°C
GPS Coordinates40.442102 N, 79.965586 W Map
Discovery NotesThe sample was collected in Pittsburgh, PA in front of a house on Robinson St. The soil was rather loose, dark, wet, and contained woodchips. When collected, the weather was 81°F at around 9:00am.
Naming NotesThe name was given as a nod to the country that I was born in, Bosnia and Herzegovina.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 25, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1772
Genome length (bp)54546
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionNyack College
Annotation StatusIn GenBank
Plaque NotesThe plaques are consistently small, clear, and circular, indicating that there is only one type of phage. The plaques were less than 2 mm in length.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Bosnia_1
(terminase small subunit)

Bosnia_2
(terminase large subunit)

Bosnia_3

Bosnia_4
(portal protein)

Bosnia_5
(capsid maturation protease)

Bosnia_6
(major capsid protein)

Bosnia_7

Bosnia_8
(head-to-tail adaptor)

Bosnia_9
(head-to-tail stopper)

Bosnia_10
(minor capsid protein)

Bosnia_11
(tail terminator)

Bosnia_12
(major tail protein)

Bosnia_13

Bosnia_14
(tail assembly chaperone)

Bosnia_15
(tail assembly chaperone)

Bosnia_16
(lipoprotein)

Bosnia_17
(tape measure protein)

Bosnia_18
(minor tail protein)

Bosnia_19
(minor tail protein)

Bosnia_20
(lysin A, L-Ala-D-Glu peptidase domain)

Bosnia_21
(lysin A, glycosyl hydrolase domain)

Bosnia_22
(holin)

Bosnia_23

Bosnia_24

Bosnia_25
(lysin B)

Bosnia_26
(minor tail protein)

Bosnia_27

Bosnia_28
(minor tail protein)

Bosnia_29
(minor tail protein)

Bosnia_30

Bosnia_31

Bosnia_32
(ribbon-helix-helix DNA binding domain protein)

Bosnia_33

Bosnia_34
(helix-turn-helix DNA binding domain protein)

Bosnia_35

Bosnia_36

Bosnia_37

Bosnia_38
(helix-turn-helix DNA binding domain protein)

Bosnia_39
(tyrosine integrase)

Bosnia_40
(phosphoribosyl transferase)

Bosnia_41
(DprA-like DNA processing chain A)

Bosnia_42
(helix-turn-helix DNA binding domain protein)

Bosnia_43
(peptidase)

Bosnia_44

Bosnia_45

Bosnia_46
(immunity repressor)

Bosnia_47
(helix-turn-helix DNA binding domain protein)

Bosnia_48

Bosnia_49
(DNA polymerase III sliding clamp)

Bosnia_50

Bosnia_51
(helix-turn-helix DNA binding domain protein)

Bosnia_52

Bosnia_53

Bosnia_54
(RecE-like exonuclease)

Bosnia_55
(RecT-like ssDNA binding protein)

Bosnia_56

Bosnia_57

Bosnia_58

Bosnia_59

Bosnia_60
(ASC-1 transcription coactivator)

Bosnia_61

Bosnia_62

Bosnia_63

Bosnia_64
(DNA methylase)

Bosnia_65
(methyltransferase)

Bosnia_66

Bosnia_67
(helix-turn-helix DNA binding domain protein)

Bosnia_68

Bosnia_69
(ADP-ribosyltransferase)

Bosnia_70

Bosnia_71
(helix-turn-helix DNA binding domain protein)

Bosnia_72

Bosnia_73
(RusA-like resolvase)

Bosnia_74

Bosnia_75

Bosnia_76
(DNA binding protein)

Bosnia_77
(MerR-like helix-turn-helix DNA binding domain protein)

Bosnia_78

Bosnia_79

Bosnia_80

Bosnia_81

Bosnia_82
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT952844
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.33x10^10
Pitt Freezer Box# 89
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload