Gordonia phage Bunnybear
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Detailed Information for Phage Bunnybear
Discovery Information
Isolation HostGordonia terrae CAG3
Found ByAnisha Reddy
Year Found2019
Location FoundPittsburgh, PA United States of America
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.409 N, 79.912 W Map
Discovery NotesFor this particular sample, the sky was overcast, and prior to collection, there was light to moderate rainfall, causing the soil to be slightly damp. The soil was dark and had a layer of mulch on top. Surrounding the area of collection, there were several shrubs and bushes, patches of tall grass, and some mature trees with moss-covered trunks. The soil was a dark brown color and appeared fine and grainy to the touch, and it was collected relatively close to the surface after scraping off some mulch and wood chips. Upon collection, some small, white bugs crawled out of the soil.
Naming NotesThe name for this phage was inspired by a nickname I was given by one of my friends.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage928
Genome length (bp)51268
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesMy phage exhibits small, circular, clear plaques with a slight turbid halo around the rim.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Bunnybear_1
(terminase small subunit)

Bunnybear_2
(terminase large subunit)

Bunnybear_3

Bunnybear_4
(portal protein)

Bunnybear_5
(capsid maturation protease)

Bunnybear_6
(major capsid protein)

Bunnybear_7

Bunnybear_8
(head-to-tail adaptor)

Bunnybear_9
(head-to-tail stopper)

Bunnybear_10
(minor capsid protein)

Bunnybear_11
(tail terminator)

Bunnybear_12
(major tail protein)

Bunnybear_13
(tail assembly chaperone)

Bunnybear_14
(tail assembly chaperone)

Bunnybear_15

Bunnybear_16
(tape measure protein)

Bunnybear_17
(minor tail protein)

Bunnybear_18
(minor tail protein)

Bunnybear_19
(lysin A, protease C39 domain)

Bunnybear_20
(lysin A, glycosyl hydrolase domain)

Bunnybear_21
(holin)

Bunnybear_22

Bunnybear_23

Bunnybear_24
(lysin B)

Bunnybear_25
(minor tail protein)

Bunnybear_26
(minor tail protein)

Bunnybear_27

Bunnybear_28
(RelB-like antitoxin)

Bunnybear_29
(RelE-like toxin)

Bunnybear_30

Bunnybear_31

Bunnybear_32
(tyrosine integrase)

Bunnybear_33
(tyrosine integrase)

Bunnybear_34
(helix-turn-helix DNA binding domain protein)

Bunnybear_35

Bunnybear_36
(metalloprotease)

Bunnybear_37
(immunity repressor)

Bunnybear_38
(helix-turn-helix DNA binding domain protein)

Bunnybear_39

Bunnybear_40

Bunnybear_41
(antirepressor)

Bunnybear_42

Bunnybear_43

Bunnybear_44

Bunnybear_45

Bunnybear_46

Bunnybear_47

Bunnybear_48

Bunnybear_49

Bunnybear_50
(dnaQ-like DNA polymerase III subunit)

Bunnybear_51

Bunnybear_52
(RecE-like exonuclease)

Bunnybear_53
(RecT-like ssDNA binding protein)

Bunnybear_54

Bunnybear_55

Bunnybear_56
(DnaJ-like chaperonin)

Bunnybear_57
(DNA methylase)

Bunnybear_58

Bunnybear_59

Bunnybear_60

Bunnybear_61
(oxidoreductase)

Bunnybear_62

Bunnybear_63
(ADP-ribosyltransferase)

Bunnybear_64

Bunnybear_65

Bunnybear_66

Bunnybear_67
(DnaC-like helicase loader)

Bunnybear_68

Bunnybear_69
(RusA-like resolvase)

Bunnybear_70

Bunnybear_71

Bunnybear_72

Bunnybear_73
(MerR-like helix-turn-helix DNA binding domain protein)

Bunnybear_74

Bunnybear_75

Bunnybear_76

Bunnybear_77

Bunnybear_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT723938
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 9 x 10^9 pfu/mL
Pitt Freezer Box# 113
Pitt Freezer Box Grid# H3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload