The Actinobacteriophage Database

Gordonia phage Bunnybear

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Detailed Information for Phage Bunnybear
Discovery Information
Isolation Host	Gordonia terrae CAG3
Found By	Anisha Reddy
Year Found	2019
Location Found	Pittsburgh, PA United States of America
Finding Institution	University of Pittsburgh
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	30°C
GPS Coordinates	40.409 N, 79.912 W Map
Discovery Notes	For this particular sample, the sky was overcast, and prior to collection, there was light to moderate rainfall, causing the soil to be slightly damp. The soil was dark and had a layer of mulch on top. Surrounding the area of collection, there were several shrubs and bushes, patches of tall grass, and some mature trees with moss-covered trunks. The soil was a dark brown color and appeared fine and grainy to the touch, and it was collected relatively close to the surface after scraping off some mulch and wood chips. Upon collection, some small, white bugs crawled out of the soil.
Naming Notes	The name for this phage was inspired by a nickname I was given by one of my friends.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 5, 2020
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	928
Genome length (bp)	51268
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGCTGGGGA
GC Content	66.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	CZ
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Bunnybear (None) Ohgeesy (None) Agueybana (CZ1) AlumE (CZ1) Antonio (CZ1) BatStarr (CZ1) Bialota (CZ1) Bosnia (CZ1) BoyNamedSue (CZ1) Eudoria (CZ1) Eviarto (CZ1) Herod (CZ1) Kita (CZ1) Madeline (CZ1) Manasvini (CZ1) Maridalia (CZ1) Neobush (CZ1) Nymphadora (CZ1) Polly (CZ1) Suscepit (CZ1) Tayonia (CZ1) ThankyouJordi (CZ1) TimTam (CZ1) Trumpet (CZ1) WelcomeAyanna (CZ1) Zameen (CZ1) Zirinka (CZ1) Attis (CZ2) Bjanes7 (CZ2) Cynthia (CZ2) Ebert (CZ2) GemG (CZ2) Gizermo (CZ2) Haley23 (CZ2) Lamberg (CZ2) LordFarquaad (CZ2) Matteo (CZ2) Mocha12 (CZ2) MrBates (CZ2) Sahara (CZ2) Savage (CZ2) SoilAssassin (CZ2) Sproutie (CZ2) TuertoX (CZ2) Vasanti (CZ2) Whiteclaw (CZ2) Yeet412 (CZ2) BaxterFox (CZ3) Yeezy (CZ3) Adora (CZ4) Beenie (CZ4) Clark (CZ4) DobbysSock (CZ4) Dolores (CZ4) Dorito (CZ4) Easley (CZ4) Hortense (CZ4) Howe (CZ4) Mcklovin (CZ4) MichaelScott (CZ4) Powerball (CZ4) Samman98 (CZ4) Sekhmet (CZ4) Shlim410 (CZ4) Suerte (CZ4) Twinkle (CZ4) WinkNick (CZ4) Denise (CZ5) Faith5x5 (CZ6) Moosehead (CZ6) Sidious (CZ7)
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	My phage exhibits small, circular, clear plaques with a slight turbid halo around the rim.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Bunnybear_1 (terminase small subunit) Bunnybear_2 (terminase large subunit) Bunnybear_3 Bunnybear_4 (portal protein) Bunnybear_5 (capsid maturation protease) Bunnybear_6 (major capsid protein) Bunnybear_7 Bunnybear_8 (head-to-tail adaptor) Bunnybear_9 (head-to-tail stopper) Bunnybear_10 (minor capsid protein) Bunnybear_11 (tail terminator) Bunnybear_12 (major tail protein) Bunnybear_13 (tail assembly chaperone) Bunnybear_14 (tail assembly chaperone) Bunnybear_15 Bunnybear_16 (tape measure protein) Bunnybear_17 (minor tail protein) Bunnybear_18 (minor tail protein) Bunnybear_19 (lysin A, protease C39 domain) Bunnybear_20 (lysin A, glycosyl hydrolase domain) Bunnybear_21 (holin) Bunnybear_22 Bunnybear_23 Bunnybear_24 (lysin B) Bunnybear_25 (minor tail protein) Bunnybear_26 (minor tail protein) Bunnybear_27 Bunnybear_28 (RelB-like antitoxin) Bunnybear_29 (RelE-like toxin) Bunnybear_30 Bunnybear_31 Bunnybear_32 (tyrosine integrase) Bunnybear_33 (tyrosine integrase) Bunnybear_34 (helix-turn-helix DNA binding domain protein) Bunnybear_35 Bunnybear_36 (metalloprotease) Bunnybear_37 (immunity repressor) Bunnybear_38 (helix-turn-helix DNA binding domain protein) Bunnybear_39 Bunnybear_40 Bunnybear_41 (antirepressor) Bunnybear_42 Bunnybear_43 Bunnybear_44 Bunnybear_45 Bunnybear_46 Bunnybear_47 Bunnybear_48 Bunnybear_49 Bunnybear_50 (dnaQ-like DNA polymerase III subunit) Bunnybear_51 Bunnybear_52 (RecE-like exonuclease) Bunnybear_53 (RecT-like ssDNA binding protein) Bunnybear_54 Bunnybear_55 Bunnybear_56 (DnaJ-like chaperonin) Bunnybear_57 (DNA methylase) Bunnybear_58 Bunnybear_59 Bunnybear_60 Bunnybear_61 (oxidoreductase) Bunnybear_62 Bunnybear_63 (ADP-ribosyltransferase) Bunnybear_64 Bunnybear_65 Bunnybear_66 Bunnybear_67 (DnaC-like helicase loader) Bunnybear_68 Bunnybear_69 (RusA-like resolvase) Bunnybear_70 Bunnybear_71 Bunnybear_72 Bunnybear_73 (MerR-like helix-turn-helix DNA binding domain protein) Bunnybear_74 Bunnybear_75 Bunnybear_76 Bunnybear_77 Bunnybear_78 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MT723938
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	9 x 10^9 pfu/mL
Pitt Freezer Box#	113
Pitt Freezer Box Grid#	H3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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