Gordonia phage Mcklovin
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Detailed Information for Phage Mcklovin
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMcKenna Vettori
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.446812 N, 79.954169 W Map
Discovery NotesPhage adopted from Matthew Weiss; sample found outside Langley Hall at the University of Pittsburgh in a moist flower bed covered in vegetation.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 19, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1881
Genome length (bp)53155
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content65.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ4
Cluster Life CycleTemperate
Lysogeny NotesEvidence towards temperate: plaques appeared cloudy at times. Some high-concentration plates in a serial dilution contained a lawn with few plaques, possibly due to the temperate cycle allowing the bacteria to grow before becoming lytic.
Other Cluster Members
Annotating InstitutionFlorida International University
Annotation StatusIn GenBank
Plaque NotesPlaques are small, toughly 2mm in diameter. Very round, and sometimes ever appear with a cloudy border possibly from temperate life cycle.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Mcklovin_1
(terminase small subunit)

Mcklovin_2

Mcklovin_3
(terminase large subunit)

Mcklovin_4

Mcklovin_5
(portal protein)

Mcklovin_6
(capsid maturation protease)

Mcklovin_7
(major capsid protein)

Mcklovin_8

Mcklovin_9
(head-to-tail adaptor)

Mcklovin_10
(head-to-tail stopper)

Mcklovin_11

Mcklovin_12
(tail terminator)

Mcklovin_13
(major tail protein)

Mcklovin_14

Mcklovin_15

Mcklovin_16
(tail assembly chaperone)

Mcklovin_17
(tail assembly chaperone)

Mcklovin_18
(tape measure protein)

Mcklovin_19
(minor tail protein)

Mcklovin_20
(minor tail protein)

Mcklovin_21
(lysin A)

Mcklovin_22

Mcklovin_23

Mcklovin_24

Mcklovin_25
(lysin B)

Mcklovin_26
(minor tail protein)

Mcklovin_27

Mcklovin_28
(minor tail protein)

Mcklovin_29
(hydrolase)

Mcklovin_30
(glycosyltransferase)

Mcklovin_31
(glycosyltransferase)

Mcklovin_32

Mcklovin_33
(helix-turn-helix DNA binding domain protein)

Mcklovin_34
(tyrosine integrase)

Mcklovin_35
(hydrolase)

Mcklovin_36
(tyrosine integrase)

Mcklovin_37
(helix-turn-helix DNA binding domain protein)

Mcklovin_38
(peptidase)

Mcklovin_39

Mcklovin_40

Mcklovin_41
(immunity repressor)

Mcklovin_42
(helix-turn-helix DNA binding domain protein)

Mcklovin_43

Mcklovin_44

Mcklovin_45
(antirepressor)

Mcklovin_46

Mcklovin_47

Mcklovin_48
(excise)

Mcklovin_49

Mcklovin_50
(DNA methylase)

Mcklovin_51

Mcklovin_52

Mcklovin_53
(RecE-like exonuclease)

Mcklovin_54
(RecT-like ssDNA binding protein)

Mcklovin_55

Mcklovin_56

Mcklovin_57

Mcklovin_58
(DNA methylase)

Mcklovin_59

Mcklovin_60

Mcklovin_61

Mcklovin_62

Mcklovin_63
(helix-turn-helix DNA binding domain protein)

Mcklovin_64

Mcklovin_65
(HNH endonuclease)

Mcklovin_66

Mcklovin_67

Mcklovin_68
(helix-turn-helix DNA binding domain protein)

Mcklovin_69

Mcklovin_70
(RusA-like resolvase)

Mcklovin_71

Mcklovin_72

Mcklovin_73

Mcklovin_74
(MerR-like helix-turn-helix DNA binding domain protein)

Mcklovin_75

Mcklovin_76

Mcklovin_77

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN428055
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.0x10^10 pfu/mL
Pitt Freezer Box# 57
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload