Gordonia phage Twinkle
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Detailed Information for Phage Twinkle
Discovery Information
Isolation HostGordonia terrae 3612
Found ByDeanna Moore
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature-10°C
GPS Coordinates40.4440088 N, 79.956054 W Map
Discovery NotesFound in a planter outside of Nordenberg Hall.
Naming NotesI let my mom name this phage because she doesn't like anything that has to do with bacteria.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 23, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage286
Genome length (bp)54241
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGCCAAGGGGA
GC Content65.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesHas small and large transparent plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Twinkle_1
(terminase small subunit)

Twinkle_2
(terminase large subunit)

Twinkle_3
(portal protein)

Twinkle_4
(capsid maturation protease)

Twinkle_5
(scaffolding protein)

Twinkle_6
(major capsid protein)

Twinkle_7

Twinkle_8
(head-to-tail adaptor)

Twinkle_9
(head-to-tail stopper)

Twinkle_10

Twinkle_11
(tail terminator)

Twinkle_12
(major tail protein)

Twinkle_13

Twinkle_14

Twinkle_15
(tail assembly chaperone)

Twinkle_16
(tail assembly chaperone)

Twinkle_17

Twinkle_18
(tape measure protein)

Twinkle_19
(minor tail protein)

Twinkle_20
(minor tail protein)

Twinkle_21
(lysin A)

Twinkle_22

Twinkle_23

Twinkle_24

Twinkle_25
(lysin B)

Twinkle_26

Twinkle_27

Twinkle_28

Twinkle_29
(minor tail protein)

Twinkle_30
(glycosyltransferase)

Twinkle_31
(glycosyltransferase)

Twinkle_32

Twinkle_33
(helix-turn-helix DNA binding protein)

Twinkle_34

Twinkle_35
(tyrosine integrase)

Twinkle_36

Twinkle_37

Twinkle_38

Twinkle_39
(tyrosine integrase)

Twinkle_40
(helix-turn-helix DNA binding protein)

Twinkle_41
(DNA binding protein)

Twinkle_42

Twinkle_43
(MerR-like helix-turn-helix DNA binding protein)

Twinkle_44
(immunity repressor)

Twinkle_45
(helix-turn-helix DNA binding protein)

Twinkle_46

Twinkle_47

Twinkle_48
(helix-turn-helix DNA binding protein)

Twinkle_49

Twinkle_50

Twinkle_51
(helix-turn-helix DNA binding protein)

Twinkle_52

Twinkle_53
(DNA methylase)

Twinkle_54

Twinkle_55

Twinkle_56
(RecE-like exonuclease)

Twinkle_57
(HNH endonuclease)

Twinkle_58
(RecT-like ssDNA binding protein)

Twinkle_59

Twinkle_60

Twinkle_61

Twinkle_62

Twinkle_63

Twinkle_64
(helix-turn-helix DNA binding protein)

Twinkle_65

Twinkle_66

Twinkle_67

Twinkle_68

Twinkle_69
(DnaB-like dsDNA helicase)

Twinkle_70
(RusA-like resolvase)

Twinkle_71

Twinkle_72

Twinkle_73

Twinkle_74
(helix-turn-helix DNA binding protein)

Twinkle_75

Twinkle_76

Twinkle_77

Twinkle_78
(DNA helicase)

Twinkle_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK878902
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.0x10^9
Date of SEA Lysate Titering Feb 20, 2017
Pitt Freezer Box# 56
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload