Gordonia phage LordFarquaad
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Detailed Information for Phage LordFarquaad
Discovery Information
Isolation HostGordonia terrae 3612
Former namesSupahhotfire
Found ByChristopher DeHaven
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.443472 N, 79.955278 W Map
Discovery NotesI discovered this phage near the WPU patio, under a tree nearby. When the sample was collected it was very wet, as it had rained the night before and was located under a large tree with minimal exposure to sunlight. The sample was dark in color composed mostly of mulch and organic matter. There was no visible sand in the sample. The sample was collected at the surface of the dirt. The ambient temperature was 29 degrees Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage678
Genome length (bp)47863
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesLord Farquuad plaques were produced by plating a serial dilution of phage particles from a spot test on G. terrae in PYCa and incubated the plate for 5 days. This plate is associated with the 10^-5 dilution. The phage consistently produces plaques which are small (approximately 1-2mm in diameter) round and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

LordFarquaad_1
(terminase small subunit)

LordFarquaad_2
(terminase large subunit)

LordFarquaad_3
(portal protein)

LordFarquaad_4
(capsid maturation protease)

LordFarquaad_5
(scaffolding protein)

LordFarquaad_6
(major capsid protein)

LordFarquaad_7

LordFarquaad_8
(head-to-tail adaptor)

LordFarquaad_9
(head-to-tail stopper)

LordFarquaad_10

LordFarquaad_11
(tail terminator)

LordFarquaad_12
(major tail protein)

LordFarquaad_13
(tail assembly chaperone)

LordFarquaad_14
(tail assembly chaperone)

LordFarquaad_15
(tape measure protein)

LordFarquaad_16
(minor tail protein)

LordFarquaad_17
(minor tail protein)

LordFarquaad_18
(lysin A)

LordFarquaad_19

LordFarquaad_20

LordFarquaad_21

LordFarquaad_22

LordFarquaad_23

LordFarquaad_24
(lysin B)

LordFarquaad_25
(minor tail protein)

LordFarquaad_26

LordFarquaad_27

LordFarquaad_28
(minor tail protein)

LordFarquaad_29

LordFarquaad_30

LordFarquaad_31

LordFarquaad_32
(BrnT-like toxin)

LordFarquaad_33

LordFarquaad_34

LordFarquaad_35
(integrase)

LordFarquaad_36

LordFarquaad_37

LordFarquaad_38
(DNA binding protein)

LordFarquaad_39

LordFarquaad_40

LordFarquaad_41
(immunity repressor)

LordFarquaad_42
(helix-turn-helix DNA binding domain protein)

LordFarquaad_43

LordFarquaad_44

LordFarquaad_45
(antirepressor)

LordFarquaad_46

LordFarquaad_47
(helix-turn-helix DNA binding domain protein)

LordFarquaad_48

LordFarquaad_49

LordFarquaad_50

LordFarquaad_51

LordFarquaad_52

LordFarquaad_53
(exonuclease)

LordFarquaad_54
(RecT-like ssDNA binding protein)

LordFarquaad_55

LordFarquaad_56

LordFarquaad_57

LordFarquaad_58

LordFarquaad_59

LordFarquaad_60

LordFarquaad_61

LordFarquaad_62

LordFarquaad_63
(helix-turn-helix DNA binding domain protein)

LordFarquaad_64

LordFarquaad_65

LordFarquaad_66

LordFarquaad_67
(HNH endonuclease)

LordFarquaad_68

LordFarquaad_69

LordFarquaad_70

LordFarquaad_71
(RusA-like resolvase)

LordFarquaad_72
(helix-turn-helix DNA binding domain protein)

LordFarquaad_73

LordFarquaad_74

LordFarquaad_75

LordFarquaad_76
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801734
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 42
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload