Gordonia phage LordFarquaad
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Detailed Information for Phage LordFarquaad
Discovery Information
Isolation HostGordonia terrae 3612
Former namesSupahhotfire
Found ByChristopher DeHaven
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.443472 N, 79.955278 W Map
Discovery NotesI discovered this phage near the WPU patio, under a tree nearby. When the sample was collected it was very wet, as it had rained the night before and was located under a large tree with minimal exposure to sunlight. The sample was dark in color composed mostly of mulch and organic matter. There was no visible sand in the sample. The sample was collected at the surface of the dirt. The ambient temperature was 29 degrees Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage678
Genome length (bp)47863
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2019)
Plaque NotesLord Farquuad plaques were produced by plating a serial dilution of phage particles from a spot test on G. terrae in PYCa and incubated the plate for 5 days. This plate is associated with the 10^-5 dilution. The phage consistently produces plaques which are small (approximately 1-2mm in diameter) round and clear.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 42
Pitt Freezer Box Grid# F1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload