| Detailed Information for Phage Bjanes7 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Linn Bjanes |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.445 N, 79.953333 W Map |
| Discovery Notes | Identified area with sprouting new plants in a flower bed near the Cathedral of Learning. There was a fresh layer of snow on top of the dirt so I brushed away the snow and dug a few inches underneath the top soil.
I dug roughly 3 inches into the earth about 2 inches from the nearest plant
Collected 1 spoonful of dirt (the ground was frozen)
Placed the soil sample into the plastic bag and sealed the bag with the zip closures
Recorded the GPS coordinates using the compass app (see below)
After roughly 5 minutes of transport, the soil sample was put into a conical tube for enrichment
Additional Notes:
GPS coordinates of the soil collection location: 40˚26’42” N 79˚57’12” W
Weather conditions at the time of the collection: strong winds, overcast with snow showers, 34 degrees F
Weather conditions in the last 24 hours: frequent snow showers, gusting winds and mostly cloudy; high 20s during the day and low 10s during the night
Soil conditions: soil was moist and mixed in with grass, mulch and bits of snow and ice. |
| Naming Notes | Last name: Bjanes and 7 because it was in sample #7 that the phage was discovered. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2827 |
| Genome length (bp) | 46042 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | A theory about the lysogeny is that because several morphologies present themselves (small and large/turbid) the phage uses both the lytic cycle and the lysogenic cycle. This is not supported by an immunity assay as of now but may be done later. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphologies are both small, clear plaques and large, turbid plaques. The large ones have haloes, hazy outer edges and a tiny, clear dot in the center. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG099941 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
